CoraLite®594-conjugated STAT2 Monoclonal antibody

STAT2 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G12C4

Cat no : CL594-66485

Synonyms

ISGF 3, P113, STAT113, STAT2



Tested Applications

Positive IF detected inRamos cells
Positive FC detected inRamos cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66485 targets STAT2 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STAT2 fusion protein Ag10168
Full Name signal transducer and activator of transcription 2, 113kDa
Calculated Molecular Weight 851 aa, 98 kDa
Observed Molecular Weight 113 kDa
GenBank Accession NumberBC051284
Gene Symbol STAT2
Gene ID (NCBI) 6773
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAT2, also named as p113, belongs to the transcription factor STAT family. It is a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IF stimulated genes, which drive the cell in an antiviral state. It also interacts with CRSP2, CRSP6, Simian virus 5 protein V, rabies virus phosphoprotein, IFNAR1 and IFNAR2. Its interaction with dengue virus NS5 inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), STAT2 is targeted for degradation. The calculated molecular weight of STAT2 is 98 kDa, but phosphorylated STAT2 is about 100-113 kDa.

Protocols

Product Specific Protocols
IF protocol for CL594 STAT2 antibody CL594-66485Download protocol
FC protocol for CL594 STAT2 antibody CL594-66485Download protocol
Standard Protocols
Click here to view our Standard Protocols