Tested Applications
| Positive WB detected in | A549 cells, Jurkat cells, mouse lung tissue, HeLa cells, PC-3 cells, rat lung tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13028-1-AP targets STAT4 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3660 Product name: Recombinant human STAT4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 399-748 aa of BC031212 Sequence: FRHLQPKEMKSSAGGKGNEGCHMVTEELHSITFETQICLYGLTIDLETSSLPVVMISNVSQLPNAWASIIWYNVSTNDSQNLVFFNNPPPATLSQLLEVMSWQFSSYVGRGLNSDQLHMLAEKLTVQSSYSDGHLTWAKFCKEHLPGKSFTFWTWLEAILDLIKKHILPLWIDGYVMGFVSKEKERLLLKDKMPGTFLLRFSESHLGGITFTWVDHSESGEVRFHSVEPYNKGRLSALPFADILRDYKVIMAENIPENPLKYLYPDIPKDKAFGKHYSSQPCEVSRPTERGDKGYVPSVFIPISTIRSDSTEPHSPSDLLPMSPSVYAVLRENLSPTTIETAMKSPYSAE Predict reactive species |
| Full Name | signal transducer and activator of transcription 4 |
| Calculated Molecular Weight | 748 aa, 86 kDa |
| Observed Molecular Weight | 86 kDa |
| GenBank Accession Number | BC031212 |
| Gene Symbol | STAT4 |
| Gene ID (NCBI) | 6775 |
| RRID | AB_2196604 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14765 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The JAK/STAT pathway is an extensive signaling pathway downstream of cytokine receptors. STATs are cytosolic proteins with a common structure consisting of an N-terminal oligomerization domain, which favors formation of STAT dimers, followed by a DNA-binding domain and a C-terminal SRC homology-2 (SH2) domain, which is involved in association between STATs and receptors[PMID:22383755]. Signal Transducer and Activator of Transcription 4 (STAT4) is a transcription factor that is activated by IL-12 signaling and promotes Th1-cell differentiation and IFN-γ production [PMID:21998209].
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for STAT4 antibody 13028-1-AP | Download protocol |
| IHC protocol for STAT4 antibody 13028-1-AP | Download protocol |
| WB protocol for STAT4 antibody 13028-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The STAT4 Polyclonal antibody (catalog #13028-1-AP) has 18 citations. These appear in journals including Nature, Annals of the Rheumatic Diseases, Bone Research, Advanced Science, Acta Pharmacologica Sinica, Free Radical Biology and Medicine, Cell Reports, Frontiers in Immunology, Journal of Agricultural and Food Chemistry, Life Sciences, International Immunopharmacology, Oncology Reports, Bioscience Reports, Diabetic Medicine, Food and Chemical Toxicology, Iran Journal of Basic Medical Sciences, Gene, and Journal of Oncology. Research fields span oncology, immunology, inflammation, fibrosis, and signaling pathways.
| Species | Application | Title |
|---|---|---|
Ann Rheum Dis A novel STAT4 gain-of-function variant defines a monogenic form of juvenile-onset systemic lupus erythematosus. | ||
Bone Res Nuclear FGF2 orchestrates phase separation-mediated rDNA chromatin architecture to control BMSCs cell fate | ||
Adv Sci (Weinh) A Unique Chimeric RNA: ERCC1-iASPP Drives Benzo[a]pyrene-Induced Lung Carcinogenesis via Dual Coding and Non-Coding Mechanisms. | ||
Acta Pharmacol Sin Bardoxolone displays potent activity against triple negative breast cancer by inhibiting the TRIP13/STAT3 circuit | ||
Free Radic Biol Med Downregulated GPX4 in salivary gland epithelial cells contributes to salivary secretion dysfunction in Sjogren's syndrome via lipid ROS/pSTAT4/AQP5 axis |
