Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
51070-2-AP targets STAT4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | signal transducer and activator of transcription 4 |
| Calculated Molecular Weight | 748 aa, 86 kDa |
| Observed Molecular Weight | 86 kDa |
| GenBank Accession Number | BC031212 |
| Gene Symbol | STAT4 |
| Gene ID (NCBI) | 6775 |
| RRID | AB_2286750 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14765 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The JAK/STAT pathway is an extensive signaling pathway downstream of cytokine receptors. STATs are cytosolic proteins with a common structure consisting of an N-terminal oligomerization domain, which favors formation of STAT dimers, followed by a DNA-binding domain and a C-terminal SRC homology-2 (SH2) domain, which is involved in association between STATs and receptors[PMID:22383755]. Signal Transducer and Activator of Transcription 4 (STAT4) is a transcription factor that is activated by IL-12 signaling and promotes Th1-cell differentiation and IFN-γ production [PMID:21998209].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for STAT4 antibody 51070-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The STAT4 Polyclonal antibody (catalog 51070-2-AP) has 2 total citations. They appear in Food Chemistry and the Journal of Immunology. The associated research fields span natural product/food chemistry (cytotoxic withanolides from Physalis ixocarpa, human WB) and immunology (MALAT1 regulation of Th-cell immunity and IL-10, mouse WB).
| Species | Application | Title |
|---|---|---|
J Immunol Malat1 Suppresses Immunity to Infection through Promoting Expression of Maf and IL-10 in Th Cells. |
Reviews
What customers say
The single review for this antibody is a 5-star rating. The reviewer used it at a 1:1000 dilution for Western Blot on primary CD4 T cells and reported that it works well, noting they have published results using this antibody. Overall, the feedback is positive, confirming reliable performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Katie (Verified Customer) (04-08-2022) | Works well for western blot. We have published with this antibody
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