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STAT5A Monoclonal antibody

STAT5A Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig, rat

Applications

WB, IF

Conjugate

Unconjugated

CloneNo.

1F10F6

Cat no : 66459-1-Ig

Synonyms

MGF, STAT5, STAT5A

Validation Data Gallery

Applications:
  • WB analysis using 66459-1-Ig

    WB analysis using 66459-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66459-1-Ig (STAT5A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of U-937 using 66459-1-Ig

    WB analysis of U-937 using 66459-1-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 66459-1-Ig (STAT5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66459-1-Ig

    IF Staining of HepG2 using 66459-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using STAT5A antibody (66459-1-Ig, Clone: 1F10F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 66459-1-Ig

    IF Staining of HepG2 using 66459-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using STAT5A antibody (66459-1-Ig, Clone: 1F10F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inRAW 264.7 cells, U-937 cells, rat spleen tissue, pig spleen tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66459-1-Ig targets STAT5A in WB, IF applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen STAT5A fusion protein Ag19546
Full Name signal transducer and activator of transcription 5A
Calculated molecular weight 794 aa, 92 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC027036
Gene symbol STAT5A
Gene ID (NCBI) 6776
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells.

Protocols

Product Specific Protocols
WB protocol for STAT5A antibody 66459-1-IgDownload protocol
IF protocol for STAT5A antibody 66459-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols