Anti-Human STAT5B Rabbit Recombinant Antibody

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• Anti-Human STAT5B Rabbit Recombinant Antibody (98830-1-RR)

STAT5B Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. 98830-1-RR

Clone No.252436D9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

STAT5A/B, STAT5

• 

  FC experiment of Jurkat using 98830-1-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Anti-Human STAT5B Rabbit RecAb (98830-1-RR, Clone: 252436D9) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of Transfected CHO-K1 using 98830-1-RR

  1x10^6 CHO-K1 (left) or STAT5B-transfected CHO-K1 (right) were intracellularly stained with 0.25 ug Anti-Human STAT5B Rabbit RecAb (98830-1-RR, Clone: 252436D9) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC (Intra) detected in: Jurkat cells, Transfected CHO-K1 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98830-1-RR targets STAT5B in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: signal transducer and activator of transcription 5B
• Calculated Molecular Weight: 90 kDa
• GenBank Accession Number: BC065227
• Gene Symbol: STAT5B
• Gene ID (NCBI): 6777
• ENSEMBL Gene ID: ENSG00000173757
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P51692
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

STAT5B belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5B carries out a dual function: signal transduction and activation of transcription. STAT5B mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different GH. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression.