STAU1 Polyclonal antibody

• Featured Product
• KD/KO Validated

STAU1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 14225-1-AP

Synonyms

STAU, Double-stranded RNA-binding protein Staufen homolog 1

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Validation Data Gallery

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  WB analysis of human brain using 14225-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14225-1-AP (STAU1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14225-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14225-1-AP (STAU1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14225-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14225-1-AP (STAU1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14225-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14225-1-AP (STAU1 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  IP experiment of mouse brain using 14225-1-AP

  IP result of anti-STAU1 (IP:14225-1-AP, 4ug; Detection:14225-1-AP 1:500) with mouse brain tissue lysate 3440ug.

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  IHC staining of mouse pancreas using 14225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14225-1-AP (STAU1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 14225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14225-1-AP (STAU1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14225-1-AP (STAU1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 14225-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using STAU1 antibody (14225-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human brain tissue, K-562 cells, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• RIP: See 4 publications below

Product Information

14225-1-AP targets STAU1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STAU1 fusion protein Ag5464
• Full Name: staufen, RNA binding protein, homolog 1 (Drosophila)
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC050432
• Gene Symbol: STAU1
• Gene ID (NCBI): 6780
• RRID: AB_2302744
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Staufen1 (STAU1)-mediated mRNA decay (SMD) degrades translationally active mRNAs that bind the double-stranded (ds)RNA binding protein STAU1 within their 3'-untranslated regions (3'UTRs) [PMID:21307942]. STAU1 can binds to stem structures and protect Dvl2 mRNA from degradation by associating with the secondary structure in the U1 region which is located at 5′ position of second U stretch in the U2 region [PMID:17510634]. It has a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site. There are two isoforms of STAU1 protein and the molecular weight of two isoforms are repectively 63 kDa and 55 kDa

Publications

Species	Application	Title
human	WB	Nature; hiCLIP reveals the in vivo atlas of mRNA secondary structures recognized by Staufen 1.; Authors - Yoichiro Sugimoto ; KD Validated
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
	RIP	Mol Cell; Gain of Additional BIRC3 Protein Functions through 3'-UTR-Mediated Protein Complex Formation.; Authors - Shih-Han Lee
human,mouse	WB,IHC,RIP	Nat Commun; Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk; Authors - Pingting Ying
human	WB	Neuron; Axonal Transport of TDP-43 mRNA Granules Is Impaired by ALS-Causing Mutations.; Authors - Nael H Alami
human	WB	Nucleic Acids Res; Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.; Authors - Evan P Booy