STAU1 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 84822-4-RR (same clone as 84822-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84822-4-RR (STAU1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84822-4-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 84822-4-RR (same clone as 84822-4-PBS)

  WB result of STAU1 antibody (84822-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAU1 transfected HepG2 cells. This data was developed using the same antibody clone with 84822-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84822-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84822-4-RR against Human STAU1 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

STAU1 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 84822-4-PBS

Clone No.242179D12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

242179D12, Double-stranded RNA-binding protein Staufen homolog 1, STAU

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Product Information

84822-4-PBS targets STAU1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5464

  Product name: Recombinant human STAU1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 227-577 aa of BC050432

  Sequence: GEGEGKSKKISKKNAAIAVLEELKKLPPLPAVERVKPRIKKKTKPIVKPQTSPEYGQGINPISRLAQIQQAKKEKEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFKVPQAQPTKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPMVPEVAQAVGVSQGHHTKDFTRAAPNPAKATVTAMIARELLYGGTSPTAETILKNNISSGHVPHGPLTRPSEQLDYLSRVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVESCHDMAALNILKLLSELDQQSTEMPRTGNGPMSVCGRC

• Full Name: staufen, RNA binding protein, homolog 1 (Drosophila)
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC050432
• Gene Symbol: STAU1
• Gene ID (NCBI): 6780
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O95793
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Staufen1 (STAU1)-mediated mRNA decay (SMD) degrades translationally active mRNAs that bind the double-stranded (ds)RNA binding protein STAU1 within their 3'-untranslated regions (3'UTRs) [PMID:21307942]. STAU1 can binds to stem structures and protect Dvl2 mRNA from degradation by associating with the secondary structure in the U1 region which is located at 5′ position of second U stretch in the U2 region [PMID:17510634]. It has a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site.