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  • KD/KO Validated

Stanniocalcin 2 Monoclonal antibody

Stanniocalcin 2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4H1E7

Cat no : 60063-1-Ig

Synonyms

stanniocalcin 2, Stanniocalcin related protein, STC 2, STC related protein, STC2, STCRP



Tested Applications

Positive WB detected inA549 cells, hTERT-RPE1 cells, T-47D cells, HEK-293 cells, HT-29 cells, HCT 116 cells, HepG2 cells, PC-3 cells, pig brain tissue
Positive IHC detected inmouse kidney tissue, human breast cancer tissue, human kidney tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

60063-1-Ig targets Stanniocalcin 2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Stanniocalcin 2 fusion protein Ag0359
Full Name stanniocalcin 2
Calculated molecular weight 33 kDa
Observed molecular weight44 kDa
GenBank accession numberBC000658
Gene symbol STC2
Gene ID (NCBI) 8614
RRIDAB_2197544
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Stanniocalcin (STC) is a family of secreted glycoprotein hormones that originally discovered in the corpuscles of Stannius, an endocrine gland of fish. STC1 and STC2, two homologues of STC family, are reported to involve in calcium and phosphate homeostasis. It is expressed in a wide variety of tissues such as kidney, spleen, heart, and pancreas. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC2 overexpression could promote tumor cell proliferation, invasion and metastasis in prostate cancer, ovarian cancer or neuroblastoma. STC2 is also vital for cytoprotective properties when exposed to ER stress and hypoxia.

Protocols

Product Specific Protocols
WB protocol for Stanniocalcin 2 antibody 60063-1-IgDownload protocol
IHC protocol for Stanniocalcin 2 antibody 60063-1-IgDownload protocol
IF protocol for Stanniocalcin 2 antibody 60063-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Hepatocell Carcinoma

The Oncogenic and Diagnostic Potential of Stanniocalcin 2 in Hepatocellular Carcinoma.

Authors - Zhixian WuJin
humanIHC, WB

Biochem Biophys Res Commun

The novel prognostic risk factor STC2 can regulate the occurrence and progression of osteosarcoma via the glycolytic pathway.

Authors - Bo Yu
  • KD Validated
humanWB

Biomed Res Int

STC2 Is a Potential Prognostic Biomarker for Pancreatic Cancer and Promotes Migration and Invasion by Inducing Epithelial-Mesenchymal Transition.

Authors - Chen Lin
  • KD Validated
humanWB

Oncotarget

STC2 promotes head and neck squamous cell carcinoma metastasis through modulating the PI3K/AKT/Snail signaling.

Authors - Shuwen Yang
  • KD Validated
mouseWB

Neuroscience

Profiling the Gene Expression and DNA Methylation in the Mouse Brain after Ischemic Preconditioning.

Authors - Menghua Cai
humanIHC

BMC Med Genomics

Osteosarcoma transcriptome data exploration reveals STC2 as a novel risk indicator in disease progression

Authors - Ziyue Wang