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  • KD/KO Validated

STK11/ LKB1 Polyclonal antibody

STK11/ LKB1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10746-1-AP

Synonyms

LKB1, PJS, serine/threonine kinase 11, STK11



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10746-1-AP targets STK11/ LKB1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STK11/ LKB1 fusion protein Ag1048
Full Name serine/threonine kinase 11
Calculated molecular weight 49 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC007981
Gene symbol STK11
Gene ID (NCBI) 6794
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers. STK11 has 2 isoforms with MW of 49 kDa and 45 kDa, and can be detected as 50-54 kDa after posttranslational modification.

Protocols

Product Specific Protocols
WB protocol for STK11/ LKB1 antibody 10746-1-APDownload protocol
IHC protocol for STK11/ LKB1 antibody 10746-1-APDownload protocol
IP protocol for STK11/ LKB1 antibody 10746-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

J Cell Mol Med

Sirt3 modulates fatty acid oxidation and attenuates cisplatin-induced AKI in mice.

Authors - Ming Li
humanWB

Thorac Cancer

Human papillomavirus 16 (HPV 16) E6 but not E7 inhibits the antitumor activity of LKB1 in lung cancer cells by downregulating the expression of KIF7.

Authors - Yue Hu
humanWB

Sci Rep

Decreased LKB1 predicts poor prognosis in Pancreatic Ductal Adenocarcinoma.

Authors - Jian-Yu Yang
mouseIP

Front Physiol

Puerarin Attenuates Diabetic Nephropathy by Promoting Autophagy in Podocytes.

Authors - Xueling Li
ratWB

Exp Cell Res

Aldolase promotes the development of cardiac hypertrophy by targeting AMPK signaling.

Authors - Yapeng Li
  • KD Validated
humanIHC

PLoS One

The LKB1 Tumor Suppressor as a Biomarker in Mouse and Human Tissues.

Authors - Nakada Yuji Y

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Bogdan (Verified Customer) (11-29-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: nerve