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  • KD/KO Validated

STK11/LKB1 Polyclonal antibody

STK11/LKB1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB,IP,IHC,ELISA

Conjugate

Unconjugated

Cat no : 10746-1-AP

Synonyms

LKB1, PJS, serine/threonine kinase 11, STK11, STK11/ LKB1



Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue, human testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10746-1-AP targets STK11/LKB1 in WB,IP,IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STK11/LKB1 fusion protein Ag1048
Full Name serine/threonine kinase 11
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC007981
Gene Symbol STK11
Gene ID (NCBI) 6794
RRIDAB_2271311
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers. STK11 has 2 isoforms with MW of 49 kDa and 45 kDa, and can be detected as 50-54 kDa after posttranslational modification.

Protocols

Product Specific Protocols
WB protocol for STK11/LKB1 antibody 10746-1-APDownload protocol
IHC protocol for STK11/LKB1 antibody 10746-1-APDownload protocol
IP protocol for STK11/LKB1 antibody 10746-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.

Authors - Qidong Li
  • KD Validated
humanWB

Nat Commun

Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.

Authors - Ziwen Wang
mouseWB

EBioMedicine

Exogenous NADPH exerts a positive inotropic effect and enhances energy metabolism via SIRT3 in pathological cardiac hypertrophy and heart failure

Authors - Ke Qian
humanWB

EMBO J

CARS senses cysteine deprivation to activate AMPK for cell survival.

Authors - Mengqiu Yuan
  • KD Validated
mouseWB

Kidney Int

Rho-associated, coiled-coil-containing protein kinase 1 regulates development of diabetic kidney disease via modulation of fatty acid metabolism.

Authors - Yosuke Nagai
ratCoIP

Aging Cell

Antagonizing peroxisome proliferator-activated receptor γ facilitates M1-to-M2 shift of microglia by enhancing autophagy via the LKB1-AMPK signaling pathway.

Authors - Juan Ji

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Bogdan (Verified Customer) (11-29-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: nerve