STK11/LKB1 Monoclonal antibody
STK11/LKB1 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1H8E4
Cat no : 66719-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells |
Positive IHC detected in | human testis tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
66719-1-Ig targets STK11/LKB1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | STK11/LKB1 fusion protein Ag13436 |
Full Name | serine/threonine kinase 11 |
Calculated molecular weight | 49 kDa |
Observed molecular weight | 45-49 kDa |
GenBank accession number | BC007981 |
Gene symbol | STK11 |
Gene ID (NCBI) | 6794 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers. STK11 has 2 isoforms with MW of 49 kDa and 45 kDa, and can be detected as 50-54 kDa after posttranslational modification.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for STK11/LKB1 antibody 66719-1-Ig | Download protocol |
IHC protocol for STK11/LKB1 antibody 66719-1-Ig | Download protocol |
IF protocol for STK11/LKB1 antibody 66719-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |