STK3 Polyclonal antibody

• Featured Product
• KD/KO Validated

STK3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 12097-1-AP

Synonyms

MST-2, MST2, MST 2, Mammalian STE20-like protein kinase 2, KRS1

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Validation Data Gallery

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  WB analysis of HeLa using 12097-1-AP

  WB result of STK3 antibody (12097-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-STK3 transfected HeLa cells.

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  WB analysis using 12097-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12097-1-AP (STK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 12097-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12097-1-AP (STK3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12097-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12097-1-AP (STK3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12097-1-AP

  Immunofluorescent analysis of Hela cells, using STK3 antibody 12097-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HeLa using 12097-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12097-1-AP (STK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A375 cells, HeLa cells, HL-60 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

12097-1-AP targets STK3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STK3 fusion protein Ag2741
• Full Name: serine/threonine kinase 3 (STE20 homolog, yeast)
• Calculated Molecular Weight: 491 aa, 56 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC010640
• Gene Symbol: STK3
• Gene ID (NCBI): 6788
• RRID: AB_2198801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Aging (Albany NY); LINC00968 can inhibit the progression of lung adenocarcinoma through the miR-21-5p/SMAD7 signal axis.; Authors - Yuxing Zhu
human	WB	Mol Cancer Ther; Peptide PDHPS1 inhibits ovarian cancer growth through disrupting YAP signaling.; Authors - Xinxing Pan
human	WB	Cancer Sci; The crosstalk between AXL and YAP promotes tumor progression through STAT3 activation in head and neck squamous cell carcinoma.; Authors - Jiayi Li
human	IF,IHC,WB	Cell Signal; Effect of STK3 on proliferation and apoptosis of pancreatic cancer cells via PI3K/AKT/mTOR pathway; Authors - Jun Chen ; KD Validated
human	WB	Molecules; Essential Oil from Pinus Koraiensis Pinecones Inhibits Gastric Cancer Cells via the HIPPO/YAP Signaling Pathway.; Authors - Yandong Zhang
sheep	WB,IHC,IF	Theriogenology; Expression of Hippo signaling pathway components in Hu sheep male reproductive tract and spermatozoa.; Authors - Guo-Min Zhang