Tested Applications
| Positive WB detected in | A375 cells, HeLa cells, HL-60 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12097-1-AP targets STK3/MST2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, canine, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2741 Product name: Recombinant human STK3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-306 aa of BC010640 Sequence: MEQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQ Predict reactive species |
| Full Name | serine/threonine kinase 3 (STE20 homolog, yeast) |
| Calculated Molecular Weight | 491 aa, 56 kDa |
| Observed Molecular Weight | 56-63 kDa |
| GenBank Accession Number | BC010640 |
| Gene Symbol | STK3 |
| Gene ID (NCBI) | 6788 |
| RRID | AB_2198801 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13188 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for STK3/MST2 antibody 12097-1-AP | Download protocol |
| IHC protocol for STK3/MST2 antibody 12097-1-AP | Download protocol |
| WB protocol for STK3/MST2 antibody 12097-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The STK3/MST2 Polyclonal antibody (Cat# 12097-1-AP) has 16 citations published in journals including Advanced Science, Cell Death & Disease, Journal for ImmunoTherapy of Cancer, EMBO Journal, Cell Mol Gastroenterol Hepatol, Molecular Cancer Therapeutics, Drug Design Development and Therapy, Aging, Molecules, Cancer Science, Cell Signaling, Life, Food and Chemical Toxicology, Theriogenology, and PLoS ONE. Research fields span cancer (pancreatic, bladder, ovarian, gastric, lung, breast, head and neck), liver disease, hepatic fibrosis, exosome-mediated regeneration, renal injury, reproductive biology, and miscarriage.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) TEAD1 Enhances Exosome Secretion and Promotes Exosome-Mediated Tissue Regeneration. | ||
Cell Death Dis Mitoguardin 1 and 2 promote granulosa cell proliferation by activating AKT and regulating the Hippo-YAP1 signaling pathway | ||
J Immunother Cancer Overcoming immunotherapy resistance in bladder cancer with a novel antibody-drug conjugate RC48 | ||
EMBO J MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression | ||
Cell Mol Gastroenterol Hepatol RASSF4 attenuates metabolic dysfunction-associated steatotic liver disease progression via Hippo signaling and suppresses hepatocarcinogenesis | ||
Mol Cancer Ther Peptide PDHPS1 inhibits ovarian cancer growth through disrupting YAP signaling. |
