STK3/MST2 Recombinant monoclonal antibody

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• STK3/MST2 Recombinant monoclonal antibody (87269-1-RR)

STK3/MST2 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87269-1-RR

Clone No.252194B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

MST2, STK3, EC:2.7.11.1, KRS1, Mammalian STE20-like protein kinase 2

• 

  WB analysis of HeLa using 87269-1-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 87269-1-RR (STK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity of 87269-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87269-1-RR against Human STK3/MST2 were performed. The affinity constant is 2.00 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87269-1-RR targets STK3/MST2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2741

  Product name: Recombinant human STK3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-306 aa of BC010640

  Sequence: MEQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQ

• Full Name: serine/threonine kinase 3 (STE20 homolog, yeast)
• Calculated Molecular Weight: 491 aa, 56 kDa
• Observed Molecular Weight: 56-63 kDa
• GenBank Accession Number: BC010640
• Gene Symbol: STK3
• Gene ID (NCBI): 6788
• RRID: AB_3745437
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13188
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protein encoded by STK3 gene is a key serine/threonine kinase, and its protein product can be cleaved by caspase in the process of apoptosis, and the inhibitory C-terminal part is removed, and the N-terminal part is transferred to the nucleus to form homodimer, which becomes an active kinase and promotes chromatin condensation in the process of apoptosis. STK3 is one of the core components of Hippo signaling pathway, which plays a key role in controlling cell proliferation, apoptosis and tissue growth. In the Hippo signaling pathway, STK3 forms a cascade reaction with STK4, LATS1/2 and other kinases, phosphorylates and activates LATS1/2, and then inhibits transcription coactivators YAP and TAZ, thus controlling cell growth and preventing cancer progression. STK3 and STK4(MST1) are similar in structure and function. They have conserved amino-terminal kinase domains, carboxyl-terminal regulatory domains and interaction domains.