STK32A Polyclonal antibody

STK32A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16223-1-AP

Synonyms

serine/threonine kinase 32A, STK32A, YANK1

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Validation Data Gallery

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  WB analysis using 16223-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16223-1-AP (STK32A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16223-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16223-1-AP (STK32A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16223-1-AP targets STK32A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STK32A fusion protein Ag9276
• Full Name: serine/threonine kinase 32A
• Calculated Molecular Weight: 396aa,46 kDa; 166aa,20 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC021666
• Gene Symbol: STK32A
• Gene ID (NCBI): 202374
• RRID: AB_2196485
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STK32A(Serine/threonine-protein kinase 32A) is also named as YANK1 and belongs to Ser/Thr protein kinase family. It can affect cell metabolism by protein serine/threonine kinase phosphorylation. The gene encoding STK32A maps to human chromosome 5 and this gene is associated with Cockayne syndrome through the ERCC8 gene and familial adenomatous polyposis through the adenomatous polyposis coli (APC) tumor suppressor gene.

Publications

Species	Application	Title
human	WB	Free Radic Biol Med; NOX1 triggers ferroptosis and ferritinophagy, contributes to Parkinson's disease; Authors - Huiqing Wang