STK32A Monoclonal antibody

STK32A Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H2A6

Cat no : 67528-1-Ig

Synonyms

serine/threonine kinase 32A, STK32A, YANK1

---

Validation Data Gallery

• 

  WB analysis of A549 using 67528-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67528-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67528-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 67528-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of JAR using 67528-1-Ig

  JAR cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 67528-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67528-1-Ig (STK32A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67528-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67528-1-Ig (STK32A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67528-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67528-1-Ig (STK32A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, MCF-7 cells, T-47D cells, JAR cells
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67528-1-Ig targets STK32A in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: STK32A fusion protein Ag10942
• Full Name: serine/threonine kinase 32A
• Calculated Molecular Weight: 396aa,46 kDa; 166aa,20 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC021666
• Gene Symbol: STK32A
• Gene ID (NCBI): 202374
• RRID: AB_2882747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.