STK36 Polyclonal antibody

STK36 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12559-1-AP

Synonyms

FU, Fused homolog, KIAA1278, STK36

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Validation Data Gallery

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  WB analysis of mouse testis using 12559-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12559-1-AP (STK36 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12559-1-AP

  IP result of anti-STK36 (IP:12559-1-AP, 4ug; Detection:12559-1-AP 1:500) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human prostate using 12559-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate using 12559-1-AP (STK36 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate using 12559-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate using 12559-1-AP (STK36 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 12559-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12559-1-AP (STK36 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12559-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12559-1-AP (STK36 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 12559-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12559-1-AP (STK36 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 12559-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12559-1-AP (STK36 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human prostate tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

12559-1-AP targets STK36 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STK36 fusion protein Ag3279
• Full Name: serine/threonine kinase 36, fused homolog (Drosophila)
• Calculated Molecular Weight: 1315 aa, 144 kDa
• Observed Molecular Weight: 144 kDa
• GenBank Accession Number: BC026158
• Gene Symbol: STK36
• Gene ID (NCBI): 27148
• RRID: AB_2196785
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STK36(Serine/threonine-protein kinase 36) is also named as KIAA1278 and belongs to the Ser/Thr protein kinase family. The Serine/threonine protein kinase plays an important role in the sonic hedgehog (Shh) pathway by regulating the activity of GLI transcription factors and controls the activity of the transcriptional regulators GLI1, GLI2 and GLI3 by opposing the effect of SUFU and promoting their nuclear localization. It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	IF	Dev Biol; Mammalian Fused is essential for sperm head shaping and periaxonemal structure formation during spermatogenesis.; Authors - Yoko Inès Nozawa
mouse	WB,IF	FASEB J; The putative protein kinase Stk36 is essential for ciliogenesis and CSF flow by associating with Ulk4; Authors - Hongye Zhang
human	WB,IHC	Sci Rep; Serine/threonine kinase 36 induced epithelial-mesenchymal transition promotes docetaxel resistance in prostate cancer; Authors - Tao He