STK38 Polyclonal antibody

STK38 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 55335-1-AP

Synonyms

NDR, NDR1, NDR1 protein kinase, Nuclear Dbf2 related kinase 1, serine/threonine kinase 38, STK38

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Validation Data Gallery

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  WB analysis of MCF-7 using 55335-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 55335-1-AP (STK38 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 55335-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 55335-1-AP (STK38 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55335-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55335-1-AP (STK38 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 55335-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55335-1-AP (STK38 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 55335-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55335-1-AP (STK38 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 55335-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55335-1-AP (STK38 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 55335-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55335-1-AP (STK38 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 55335-1-AP

  Immunofluorescent analysis of HEK-293 cells using 55335-1-AP (STK38 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells
• Positive IHC detected in: human kidney tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

55335-1-AP targets STK38 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: serine/threonine kinase 38
• Calculated molecular weight: 54 kDa
• Observed molecular weight: 54 kDa
• GenBank accession number: NM_007271
• Gene symbol: STK38
• Gene ID (NCBI): 11329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serine/threonine-protein kinase 38 belongs to AGC Ser/Thr protein kinase family.STK38 implicates in cell proliferation and/or tumor progression, and might play a role in the HIV-1 life cycle.Researches showed that STK38 with STK38L, were proapoptotic kinases and key members of the RASSF1/STK4 signaling cascade and its kinase activity increased in early mitotic phase and was dependent on FRY and STK3.This antibody recognizes the C-terminal of STK38.

Publications

Species	Application	Title
human	IF, WB, IHC	J Cell Biochem; Phosphorylated LASS2 inhibits prostate carcinogenesis via negative regulation of Wnt/β-catenin signaling.; Authors - Kuangen Zhang
human	WB,IF	J Proteome Res; ZSWIM1 Promotes the Proliferation and Metastasis of Lung Adenocarcinoma Cells through the STK38/MEKK2/ERK1/2 Axis; Authors - Xuejuan Gao
human	WB,IP	Nat Commun; A bacterial autotransporter impairs innate immune responses by targeting the transcription factor TFE3; Authors - Atri Ta