Stathmin 1 Monoclonal antibody

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• KD/KO Validated

Stathmin 1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

7D1E1

Cat no : 66090-1-Ig

Synonyms

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Validation Data Gallery

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  WB analysis of Jurkat using 66090-1-Ig

  WB result of Stathmin antibody (66090-1-Ig, 1:4000) with si-Control and si-Stathmin transfected Jurkat cells.

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  WB analysis of Jurkat using 66090-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66090-1-Ig (Stathmin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66090-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66090-1-Ig (Stathmin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 66090-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66090-1-Ig (Stathmin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 66090-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66090-1-Ig (Stathmin antibody at dilution of 1:50.

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  IF Staining of mouse olfactory epithelium tissue using 66090-1-Ig

  Fluorescent IHC on 1%PLP fixed frozen mouse olfactory epithelium tissue of STMN1 antibody (66090-1-IG, 1:200). Basal cell staining and dendritic processes as expected. By Dr. Brian Lin (Schwob Lab).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, human brain tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse olfactory epithelium tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66090-1-Ig is Stathmin 1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Stathmin 1 fusion protein Ag19102
• Full Name: stathmin 1/oncoprotein 18
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC014353
• Gene Symbol: STMN1
• Gene ID (NCBI): 3925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Stathmin 1 (STMN1) normally regulates microtubule dynamics either by sequestering free tubulin heterodimers or by promoting microtubule catastrophe. STMN1 is highly expressed in fetal and adult brain, spinal cord, and cerebellum. Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. Phosphorylation of stathmin is involved in response to NGF, neuron polarization and microtubule polymerization inhibition activity. Increased expression of STMN1 has been observed in a variety of human malignancies, such as colorectal primary tumors and metastatic tissues, but its association with melanoma is so far not well known.