Various lysates were subjected to SDS PAGE followed by western blot with RMX00021 (STMN2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with RMX00021 (STMN2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
IP experiment of Neuro-2a using RMX00021
IP result of anti-STMN2 (IP:RMX00021, 4ug; Detection:RMX00021 1:20000) with Neuro-2a cells lysate 1440 ug.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00021 (STMN2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of SH-SY5Y using RMX00021
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using STMN2 antibody (RMX00021) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using STMN2 antibody (RMX00021) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
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mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
SH-SY5Y cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:200-1:800
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
RMX00021 targets STMN2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)
Various lysates were subjected to SDS PAGE followed by western blot with RMX00021 (STMN2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using RMX00021
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00021 (STMN2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using RMX00021
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00021 (STMN2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of Neuro-2a using RMX00021
IP result of anti-STMN2 (IP:RMX00021, 4ug; Detection:RMX00021 1:20000) with Neuro-2a cells lysate 1440 ug.
IF/ICC Figures
IF Staining of SH-SY5Y using RMX00021
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using STMN2 antibody (RMX00021) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
STMN2 Multi-Recombinant antibody
Catalog Number
RMX00021
Citations
-
Dilutions
WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:200-1:800 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
