Multi-rAb™ STMN2 Multi-Recombinant antibody

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  WB analysis using RMX00021

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00021 (STMN2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using RMX00021

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00021 (STMN2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using RMX00021

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00021 (STMN2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using RMX00021

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using STMN2 antibody (RMX00021) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

STMN2 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. RMX00021

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SCG 10, SCG10, SCGN 10, SCGN10, SGC 10

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, Neuro-2a cells, PC-12 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00021 targets STMN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: stathmin-like 2
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 18-21 kDa
• GenBank Accession Number: BC006302
• Gene Symbol: STMN2
• Gene ID (NCBI): 11075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: Q93045
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.