STMN4 Polyclonal antibody

STMN4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12027-1-AP

Synonyms

RB3, Stathmin 4, stathmin like 4, Stathmin like protein B3, STMN4

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Validation Data Gallery

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  WB analysis of human brain using 12027-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12027-1-AP (STMN4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 12027-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12027-1-AP (STMN4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12027-1-AP

  IP result of anti-STMN4 (IP:12027-1-AP, 3ug; Detection:12027-1-AP 1:1000) with mouse brain tissue lysate 6000ug.

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  IHC staining of human gliomas using 12027-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12027-1-AP (STMN4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 12027-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12027-1-AP (STMN4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 12027-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12027-1-AP (STMN4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 12027-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12027-1-AP (STMN4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 12027-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12027-1-AP (STMN4 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

12027-1-AP targets STMN4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STMN4 fusion protein Ag2657
• Full Name: stathmin-like 4
• Calculated Molecular Weight: 216 aa, 25 kDa
• Observed Molecular Weight: 22-30 kDa
• GenBank Accession Number: BC011520
• Gene Symbol: STMN4
• Gene ID (NCBI): 81551
• RRID: AB_2197401
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RB3 (Stathmin-4), encoded by STMN4 gene, is a neuron-specific homologue of the SCG10/stathmin family proteins, possessing a unique N-terminal membrane-associated domain and the stathmin-like domain at the C terminus, which promotes microtubule (MT) catastrophe and/or tubulin sequestering. Stathmin is a ubiquitous cytosolic phosphoprotein, preferentially expressed in the nervous system, and described as a relay integrating diverse intracellular signaling pathways. RB3 is consistently up-regulated in differentiated neuroblastoma cells, and stable expression of transfected RB3 could induce differentiation, suggesting a role of RB3 during neuroblastoma differentiation. Moreover, increased RB3 is a unique response protein in the stathmin-related proteins following optical nerve axotomy and RB3 may be a critical target for neurodegenerative diseases. STMN4 has 4 isoforms with MW 25, 23, 22 and 19 kDa (refer to UniProt). Another 30 kDa isoform can be found by NCBI Protein Blast. (https://www.ncbi.nlm.nih.gov/protein/XP_005273709)

Publications

Species	Application	Title
mouse	WB	Cell Rep; Loss of Stathmin-2, a hallmark of TDP-43-associated ALS, causes motor neuropathy.; Authors - Kelsey L Krus
	IF	Genome Med; Spatial transcriptomic analysis of Sonic hedgehog medulloblastoma identifies that the loss of heterogeneity and promotion of differentiation underlies the response to CDK4/6 inhibition; Authors - Tuan Vo
mouse	WB	J Cell Sci; SCG10 is required for peripheral axon maintenance and regeneration in mice; Authors - Yuanjun Li