Stomatin Polyclonal antibody

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• Stomatin Polyclonal antibody (12046-1-AP)

Stomatin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12046-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

STOM, BND7, EPB7, EPB72, Erythrocyte membrane protein band 7.2

• 

  WB analysis of HeLa using 12046-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12046-1-AP (Stomatin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 12046-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12046-1-AP (STOM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human lung using 12046-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12046-1-AP (STOM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 12046-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12046-1-AP (STOM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A549 using 12046-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12046-1-AP (Stomatin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 12046-1-AP

  IP result of anti-Stomatin (IP:12046-1-AP, 3ug; Detection:12046-1-AP 1:300) with HeLa cells lysate 2800ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 12046-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12046-1-AP (Stomatin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 12046-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12046-1-AP (Stomatin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 12046-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Stomatin antibody (12046-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, human liver tissue, human lung tissue, K-562 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12046-1-AP targets Stomatin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2676

  Product name: Recombinant human STOM protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-288 aa of BC010703

  Sequence: MAEKRHTRDSEAQRLPDSFKDSPSKGLGPCGWILVAFSFLFTVITFPISIWMCIKIIKEYERAIIFRLGRILQGGAKGPGLFFILPCTDSFIKVDMRTISFDIPPQEILTKDSVTISVDGVVYYRVQNATLAVANITNADSATRLLAQTTLRNVLGTKNLSQILSDREEIAHNMQSTLDDATDAWGIKVERVEIKDVKLPVQLQRAMAAEAEASREARAKVIAAEGEMNASRALKEASMVITEYPAALQLRYLQTLTTIAAEKNSTIVFPLPIDMLQGIIGAKHSHLG

• Full Name: stomatin
• Calculated Molecular Weight: 288 aa, 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC010703
• Gene Symbol: Stomatin
• Gene ID (NCBI): 2040
• RRID: AB_2197406
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P27105
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STOM, also named as BND7, EPB72, Stomatin and Protein 7.2b, belongs to the band 7/mec-2 family. It is thought to regulate cation conductance. STOM may regulate ACCN1 and ACCN3 gating. STOM is a membrane protein involved in regulation of monovalent cation transport through lipid membranes. Interestingly, STOM (which has a structure similar to caveolin) is a major lipid-raft component of erythrocytes. It may have a role in membrane microdomains modulation leading to membrane budding and MPs release.(PMID:19138258)

Publications

What published studies show

The Stomatin Polyclonal antibody (Cat# 12046-1-AP) has 8 citations across journals including J Biol Chem, BMC Cancer, Biophys J, Front Med, Front Oncol, Chin J Cancer Res, Curr Eye Res, and Vox Sang. Research fields span cancer biology (NSCLC metastasis, osteosarcoma, orbital DLBCL), membrane biophysics (proton channel–cholesterol interactions), red blood cell biology (deformation, microparticles), diabetic retinopathy angiogenesis, and motor protein complex assembly.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Chin J Cancer Res; Stomatin plays a suppressor role in non-small cell lung cancer metastasis.; Authors - Huaying An
		J Biol Chem; Association of protein arginine deiminase 4 with the myosin-9 motor complex; Authors - Xiaoxing Wang
human	IHC	BMC Cancer; Role of arachidonic acid metabolism in osteosarcoma prognosis by integrating WGCNA and bioinformatics analysis; Authors - Yaling Wang
human	WB,CoIP	Biophys J; Interaction with stomatin directs human proton channels into cholesterol-dependent membrane domains; Authors - Artem G Ayuyan
human	WB	Front Med; Facile discovery of red blood cell deformation and compromised membrane/skeleton assembly in Prader-Willi syndrome; Authors - Yashuang Yang
human	WB,IHC	Front Oncol; Upregulation of stomatin is associated with poor prognosis and promotes tumor progression of orbital diffuse large B-cell lymphoma; Authors - Wen Chen