STRA6 Polyclonal antibody

STRA6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

10

Reviews (1)

Cat no : 22001-1-AP

Synonyms

MCOPS9, PP14296, STRA6, UNQ3126/PRO10282/PRO19578

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Validation Data Gallery

• 

  WB analysis using 22001-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22001-1-AP (STRA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22001-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22001-1-AP (STRA6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse kidney tissue, rat kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

22001-1-AP targets STRA6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STRA6 fusion protein Ag17591
• Full Name: stimulated by retinoic acid gene 6 homolog (mouse)
• Calculated Molecular Weight: 667 aa, 74 kDa
• Observed Molecular Weight: 73-78 kDa
• GenBank Accession Number: BC025256
• Gene Symbol: STRA6
• Gene ID (NCBI): 64220
• RRID: AB_11064606
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STRA6, also known as MCOPS9 and PP14296. Functions as retinol transporter. Accepts all-trans retinol from the extracellular retinol-binding protein RBP4, facilitates retinol transport across the cell membrane, and then transfers retinol to the cytoplasmic retinol-binding protein RBP1. Contributes to the activation of a signaling cascade that depends on retinol transport and LRAT-dependent generation of retinol metabolites that then trigger activation of JAK2 and its target STAT5, and ultimately increase the expression of SOCS3 and inhibit cellular responses to insulin. (PMID:9452451; 18316031; 22665496; 21368206; 22665496).

Publications

Species	Application	Title
mouse	WB	J Cachexia Sarcopenia Muscle; RBP4 promotes denervation-induced muscle atrophy through STRA6-dependent pathway; Authors - Kang-Zhen Zhang
mouse	IF,WB	Int J Mol Sci; Retinoid Regulation of Ocular Surface Innate Inflammation.; Authors - Jehan Alam
human	IF	Sci Rep; TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.; Authors - S Prpar Mihevc
human	WB	Cancers (Basel); ANLN Promotes the Proliferation and Migration of Gallbladder Cancer Cells via STRA6-Mediated Activation of PI3K/AKT Signaling; Authors - Xiang Zhu
human	IF	Endocrinology; De novo-synthesized retinoic acid in ovarian antral follicles enhances FSH-mediated ovarian follicular cell differentiation and female fertility.; Authors - Tomoko Kawai
mouse	IHC	Lab Invest; Nuclear receptor binding factor 2 (NRBF2) is required for learning and memory.; Authors - Xiaosen Ouyang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christian (Verified Customer) (02-19-2020)

The antibody works quite well, not many unspecific bands.

• Applications: Western Blot,
• Cell Tissue Type: HUVEC