STRBP Polyclonal antibody

STRBP Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17362-1-AP

Synonyms

SPNR, Spermatid perinuclear RNA-binding protein, p74, ILF3L

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Validation Data Gallery

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  WB analysis using 17362-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17362-1-AP (STRBP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 17362-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17362-1-AP (STRBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 17362-1-AP

  IP result of anti-STRBP (IP:17362-1-AP, 4ug; Detection:17362-1-AP 1:500) with PC-3 cells lysate 4000ug.

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  IHC staining of human ovary cancer using 17362-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17362-1-AP (STRBP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 17362-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17362-1-AP (STRBP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 17362-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17362-1-AP (STRBP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 17362-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17362-1-AP (STRBP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse testis tissue, PC-3 cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human stomach tissue, human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IP: See 1 publications below

Product Information

17362-1-AP targets STRBP in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STRBP fusion protein Ag10720
• Full Name: spermatid perinuclear RNA binding protein
• Calculated Molecular Weight: 672 aa, 74 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC017732
• Gene Symbol: STRBP
• Gene ID (NCBI): 55342
• RRID: AB_2271270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IP	J Biol Chem; Satellite double-stranded RNA induces mesenchymal transition in pancreatic cancer by regulating alternative splicing; Authors - Takuma Iwata