STUB1 Polyclonal antibody

• Featured Product
• KD/KO Validated

STUB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

28

Cat no : 55430-1-AP

Synonyms

CHIP, EC:2.3.2.27, E3 ubiquitin-protein ligase CHIP, CLL-associated antigen KW-8, Carboxy terminus of Hsp70-interacting protein

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Validation Data Gallery

• 

  WB analysis of HeLa cells using 55430-1-AP

  WB result of STUB1 antibody (55430-1-AP, 1:500) with si-control and si-STUB1 transfected HeLa cells.

• 

  WB analysis of mouse liver using 55430-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55430-1-AP (STUB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55430-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55430-1-AP (STUB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 55430-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 55430-1-AP (STUB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55430-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55430-1-AP (STUB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 55430-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55430-1-AP (STUB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 55430-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55430-1-AP (STUB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 55430-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55430-1-AP (STUB1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse skeletal muscle using 55430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55430-1-AP (STUB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 55430-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55430-1-AP (STUB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55430-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55430-1-AP (STUB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55430-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55430-1-AP (STUB1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, HEK-293 cells, MCF-7 cells, mouse liver tissue
• Positive IHC detected in: human colon cancer tissue, human liver tissue, mouse skeletal muscle tissue, human heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 26 publications below
• IHC: See 3 publications below
• IF: See 6 publications below

Product Information

55430-1-AP targets STUB1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: STIP1 homology and U-box containing protein 1
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_005861
• Gene Symbol: STUB1
• Gene ID (NCBI): 10273
• RRID: AB_10949225
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STUB1, also named as CHIP, SDCCAG7, UBOX1 and NY-CO-7, is a E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. STUB1/CHIP modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. It mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. STUB1/CHIP mediates polyubiquitination of CYP3A4.(PMID:10330192) CHIP/STUB1 strongly reduced keratin aggregates through increased degradation of mutant K14. (PMID:20151404) STUB1/CHIP regulates NAD(P)H:quinone oxidoreductase 1 (NQO1) accumulation in aged brain, a process impaired in certain Alzheimer patients.(PMID:21220432 ) This antibody is specific to STUB1.

Publications

Species	Application	Title
human	WB	Cell; PMID: 25594175; Authors - Yiqing Yan ; KD Validated
human	WB	Nat Commun; The LINC01138 drives malignancies via activating arginine methyltransferase 5 in hepatocellular carcinoma.; Authors - Zhe Li
human	IF	Nat Commun; Cardiomyocyte contractile impairment in heart failure results from reduced BAG3-mediated sarcomeric protein turnover.; Authors - Thomas G Martin
human	WB	Mol Cancer; Exosomal circSHKBP1 promotes gastric cancer progression via regulating the miR-582-3p/HUR/VEGF axis and suppressing HSP90 degradation.; Authors - Mengyan Xie
human	WB	Cell Death Dis; ZC3H15 promotes glioblastoma progression through regulating EGFR stability.; Authors - Jianbing Hou
human	WB	Oncogene; AKIP1 accelerates glioblastoma progression through stabilizing EGFR expression; Authors - Sicheng Wan

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