Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68407-1-Ig targets STUB1 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | STIP1 homology and U-box containing protein 1 |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | NM_005861 |
| Gene Symbol | STUB1 |
| Gene ID (NCBI) | 10273 |
| RRID | AB_3085124 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UNE7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
STUB1, also named as CHIP, SDCCAG7, UBOX1 and NY-CO-7, is a E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. STUB1/CHIP modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. It mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. STUB1/CHIP mediates polyubiquitination of CYP3A4.(PMID:10330192) CHIP/STUB1 strongly reduced keratin aggregates through increased degradation of mutant K14. (PMID:20151404) STUB1/CHIP regulates NAD(P)H:quinone oxidoreductase 1 (NQO1) accumulation in aged brain, a process impaired in certain Alzheimer patients.(PMID:21220432 ) This antibody is specific to STUB1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for STUB1 antibody 68407-1-Ig | Download protocol |
| WB protocol for STUB1 antibody 68407-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The STUB1 Monoclonal antibody (Cat# 68407-1-Ig) has 14 citations. Journals include Nature Communications, Journal of Advanced Research, Molecular Cell, Science Translational Medicine, Cell Death & Disease, Clinical and Translational Medicine, Cell Reports, BBA Molecular Basis of Disease, European Journal of Medical Research, Journal of Virology, Toxicology and Applied Pharmacology, Veterinary Microbiology, and Translational Research. Research fields span protein ubiquitination/degradation, cancer (gastric, breast, head and neck, leukemia), renal fibrosis, neurodegeneration/cognitive dysfunction, viral infections, mitophagy, ferroptosis, and immune regulation.
| Species | Application | Title |
|---|---|---|
Nat Commun Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha | ||
J Adv Res OTUD7B attenuates renal fibrosis by regulating PRDX1 protein stability.
| ||
Mol Cell Global profiling of nascent chain interactors reveals TRIM25 as a co-translational E3 ubiquitin ligase.
| ||
Sci Transl Med A peptide immunomodulator activates MST1 to expand and stabilize murine and human regulatory T cells for immune tolerance | ||
Cell Death Dis A new candidate tumor suppressor tRF-Ser inhibits gastric cancer progression by regulating the CNBP/HSPA8 axis. | ||
Clin Transl Med USP20 competitively binds to STUB1 to enhance CTSL expression and promote epithelial-mesenchymal transition in head and neck squamous cell carcinoma.
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Reviews
What customers say
The single review for this antibody rates it 5 stars. The user performed a Western Blot at a 1:2,000 dilution on HEK293T total cell lysates and reported that the antibody successfully detected endogenous STUB1 at the expected 35 kDa molecular weight. Overall, the reviewer described it as a good antibody with reliable performance for detecting STUB1 by Western Blot.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Christine (Verified Customer) (08-05-2025) | Detects endogenous STUB1 (35 kDa) in HEK293T total cell lysates. Good antibody
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