Syntaxin 11 Polyclonal antibody

• Featured Product
• KD/KO Validated

Syntaxin 11 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 13301-1-AP

Synonyms

Syntaxin-11, STX11

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Validation Data Gallery

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  WB analysis using 13301-1-AP

  Jurkat cells lysates were subjected to SDS PAGE followed by western blot with 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13301-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 13301-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 13301-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 13301-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 13301-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of DU 145 using 13301-1-AP

  IP result of anti-Syntaxin 11 (IP:13301-1-AP, 4ug; Detection:13301-1-AP 1:1000) with DU 145 cells lysate 1280 ug.

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  IHC staining of human lymphoma using 13301-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 13301-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse lung tissue, DU 145 cells, human placenta tissue, human skeletal muscle tissue, Jurkat cells, L02 cells, mouse placenta tissue, rat lung tissue
• Positive IP detected in: DU 145 cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

13301-1-AP targets Syntaxin 11 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Syntaxin 11 fusion protein Ag4097
• Full Name: syntaxin 11
• Calculated Molecular Weight: 287 aa, 33 kDa
• Observed Molecular Weight: 33-35 kDa
• GenBank Accession Number: BC033519
• Gene Symbol: Syntaxin 11
• Gene ID (NCBI): 8676
• RRID: AB_2198214
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin 11 (STX11) belongs to the syntaxin family. It interacts with the SNARE proteins SNAP-23 and VAMP. STX11 behaves as an integral membrane protein despite the lack of a traditional transmembrane domain. The detection of STX11 in human cytotoxic lymphocytes suggests that this protein could play a role in the process leading to degranulation of secretory lysosomes (PMID:17525286).

Publications

Species	Application	Title
human	WB,IF	J Extracell Vesicles; Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion; Authors - Chuqi Liu ; KO Validated
human	WB	J Exp Med; Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency.; Authors - Frank Cichocki
rat	WB,IF	Proc Natl Acad Sci U S A; Crucial role of the hydrophobic pocket region of Munc18 protein in mast cell degranulation.; Authors - Bin Na-Ryum NR
human	WB,IF, IP	J Cell Biol; The septin cytoskeleton regulates natural killer cell lytic granule release.; Authors - Prasad V Phatarpekar
human	WB, IHC	J Cell Sci; Chaperoning of closed syntaxin-3 through Lys46 and Glu59 in domain-1 of Munc18 is indispensable for mast cell exocytosis.; Authors - Na-Ryum Bin
human	WB	Front Immunol; Bi-Allelic Mutations in STXBP2 Reveal a Complementary Role for STXBP1 in Cytotoxic Lymphocyte Killing.; Authors - Jamie A Lopez

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