Syntaxin 12 Recombinant antibody

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  WB analysis using 85069-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85069-4-RR (Syntaxin 12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 85069-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Syntaxin 12 antibody (85069-4-RR, Clone: 242546H2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85069-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85069-4-RR against Human Syntaxin 12 were performed. The affinity constant is 1.05 nM.

Syntaxin 12 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85069-4-RR

Clone No.242546H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Syntaxin-12, STX12, 242546H2

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Tested Applications

• Positive WB detected in: LNCaP cells, HepG2 cells, HEK-293 cells, Jurkat cells, human placenta tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85069-4-RR targets Syntaxin 12 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Syntaxin 12 fusion protein Ag5532
• Full Name: syntaxin 12
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC046999
• Gene Symbol: Syntaxin 12
• Gene ID (NCBI): 23673
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q86Y82
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin 12 (STX12), also known as STX13 or STX14, is a 276 amino acid single-pass type IV membrane protein that belongs to the syntaxin family. It is a broadly and differentially expressed SNARE protein, highly enriched in brain tissue. STX12 is localized to early and recycling endosomes and is involved in the recycling of plasma membrane proteins. It also has a role in the trafficking of MMP during the degradation of ECM substrates and subsequent cellular invasion. (PMID: 9507000; 9553086; 19910495) This antibody recognizes a major 39 kDa band of endogenous STX12. In some cases, two additional bands of 33 kDa and 66 kDa could also be detected, which most likely represent a degradation product and a SNARE complex respectively (PMID: 9817754).