Syntaxin 3 Polyclonal antibody

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• Syntaxin 3 Polyclonal antibody (15556-1-AP)

Syntaxin 3 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 15556-1-AP Copied!

Publications (24)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

STX3, STX3A, Syntaxin-3

• 

  WB analysis of SH-SY5Y using 15556-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15556-1-AP (Syntaxin 3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human plasma using 15556-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 15556-1-AP (Syntaxin 3 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Neuro-2a using 15556-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 15556-1-AP (Syntaxin 3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of SH-SY5Y using 15556-1-AP

  IP result of anti-Syntaxin 3 (IP:15556-1-AP, 4ug; Detection:15556-1-AP 1:1000) with SH-SY5Y cells lysate 3600ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 15556-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15556-1-AP (Syntaxin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 15556-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15556-1-AP (Syntaxin 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, human plasma tissue, Neuro-2a cells
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15556-1-AP targets Syntaxin 3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7925

  Product name: Recombinant human STX3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-264 aa of BC007405

  Sequence: MKDRLEQLKAKQLTQDDDTDAVEIAIDNTAFMDEFFSEIEETRLNIDKISEHVEEAKKLYSIILSAPIPEPKTKDDLEQLTTEIKKRANNVRNKLKSMEKHIEEDEVRSSADLRIRKSQHSVLSRKFVEVMTKYNEAQVDFRERSKGRIQRQLEITGKKTTDEELEEMLESGNPAIFTSGIIDSQISKQALSEIEGRHKDIVRLESSIKELHDMFMDIAMLVENQGEMLDNIELNVMHTVDHVEKARDETKKAVKYQSQARKKL

• Full Name: syntaxin 3
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC007405
• Gene Symbol: STX3
• Gene ID (NCBI): 6809
• RRID: AB_2198667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13277
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin-3 (also known as STX3) is a member of the syntaxin. Syntaxin-3 is a significant apical targeting protein in the epithelial membrane and in exocytosis process; it also acts as a vesicle transporter by cellular receptor in neutrophils, which is crucial for protein trafficking event (PMID: 25630102). It has been reported that syntaxin-3 has an important role in the growth of neurites, and serves as a direct target for omega-6 arachidonic acid (PMID: 1659826).

Publications

What published studies show

The Syntaxin 3 Polyclonal antibody (Cat# 15556-1-AP) has 24 total citations. Citations appear in journals including Nature Communications, Science Advances, PNAS, Cell Reports, PLoS Biology, Molecular Therapy, Journal of Biological Chemistry, Journal of Immunology, and Scientific Reports. Research fields span photoreceptor cilia biology, SNARE complex assembly and neurotransmitter release, protein trafficking, Bardet-Biedl syndrome, exocytosis, immune cell degranulation, and inflammatory pain signaling.

Automatically generated from the 24 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IP	Nat Commun; The tectonic complex regulates membrane protein composition in the photoreceptor cilium; Authors - Hanh M Truong
human	WB	Sci Adv; Phosphorylation of Doc2 by EphB2 modulates Munc13-mediated SNARE complex assembly and neurotransmitter release; Authors - Hong Zhang
mouse	WB,IF	Cell Death Dis; ZNRF1 deficiency disrupts Fas ligand trafficking and immune balance.; Authors - Ting-Yu Lai
human	WB,IP	Mol Ther; circMRPS35 promotes malignant progression and cisplatin resistance in hepatocellular carcinoma; Authors - Peng Li
mouse	WB,IF	Proc Natl Acad Sci U S A; Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.; Authors - Poppy Datta
rat	WB	Cell Rep; Structural and Functional Analysis of the CAPS SNARE-Binding Domain Required for SNARE Complex Formation and Exocytosis.; Authors - Hao Zhou