STX3 Monoclonal antibody

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• STX3 Monoclonal antibody (66760-1-Ig)

STX3 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66760-1-Ig Copied!

Clone No. 1B9C7

Publications (1)

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Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

STX3, STX3A, syntaxin 3

• 

  WB analysis using 66760-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 66760-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 66760-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of SH-SY5Y using 66760-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat brain using 66760-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66760-1-Ig (STX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 66760-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66760-1-Ig (STX3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 66760-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66760-1-Ig (STX3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HSC-T6 cells, rat brain tissue, SH-SY5Y cells, NIH/3T3 cell, RAW 264.7 cell, HepG2 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66760-1-Ig targets STX3 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7805

  Product name: Recombinant human STX3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-264 aa of BC007405

  Sequence: MKDRLEQLKAKQLTQDDDTDAVEIAIDNTAFMDEFFSEIEETRLNIDKISEHVEEAKKLYSIILSAPIPEPKTKDDLEQLTTEIKKRANNVRNKLKSMEKHIEEDEVRSSADLRIRKSQHSVLSRKFVEVMTKYNEAQVDFRERSKGRIQRQLEITGKKTTDEELEEMLESGNPAIFTSGIIDSQISKQALSEIEGRHKDIVRLESSIKELHDMFMDIAMLVENQGEMLDNIELNVMHTVDHVEKARDETKKAVKYQSQARKKL

• Full Name: syntaxin 3
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC007405
• Gene Symbol: STX3
• Gene ID (NCBI): 6809
• RRID: AB_2882106
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q13277
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin-3 (also known as STX3) is a member of the syntaxin. Syntaxin-3 is a significant apical targeting protein in the epithelial membrane and in exocytosis process; it also acts as a vesicle transporter by cellular receptor in neutrophils, which is crucial for protein trafficking event (PMID: 25630102). It has been reported that syntaxin-3 has an important role in the growth of neurites, and serves as a direct target for omega-6 arachidonic acid (PMID: 1659826).

Publications

What published studies show

The STX3 Monoclonal antibody (1B9C7), catalog number 66760-1-Ig, has 1 citation to date. It was used in a study published in Cancer Med (2023) investigating STX3 transcript and protein levels in esophageal squamous cell carcinoma patients. The research field is oncology, specifically esophageal cancer prognosis. The antibody was applied via IHC and WB in human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC,WB	Cancer Med; Increased STX3 transcript and protein levels were associated with poor prognosis in two independent cohorts of esophageal squamous cell carcinoma patients; Authors - Takahiro Shinozuka