Tested Applications
| Positive WB detected in | A431 cells, HEK-293 cells, HepG2 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26711-1-AP targets STX5 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25098 Product name: Recombinant human STX5 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 55-303 aa of BC012137 Sequence: MSCRDRTQEFLSACKSLQTRQNGIQTNKPALRAVRQRSEFTLMAKRIGKDLSNTFAKLEKLTILAKRKSLFDDKAVEIEELTYIIKQDINSLNKQIAQLQDFVRAKGSQSGRHLQTHSNTIVVSLQSKLASMSNDFKSVLEVRTENLKQQRSRREQFSRAPVSALPLAPNHLGGGAVVLGAESHASKDVAIDMMDSRTSQQLQLIDEQDSYIQSRADTMQNIESTIVELGSIFQQLAHMVKEQEETIQR Predict reactive species |
| Full Name | syntaxin 5 |
| Calculated Molecular Weight | 32 kDa, 34 kDa, 42 kDa |
| Observed Molecular Weight | 35 and 42 kDa |
| GenBank Accession Number | BC012137 |
| Gene Symbol | STX5 |
| Gene ID (NCBI) | 6811 |
| RRID | AB_2880609 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13190 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for STX5 antibody 26711-1-AP | Download protocol |
| IHC protocol for STX5 antibody 26711-1-AP | Download protocol |
| WB protocol for STX5 antibody 26711-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The STX5 polyclonal antibody (catalog number 26711-1-AP) has 3 total citations. These appear in the Journal of Biological Chemistry, bioRxiv, and Molecular Cell. The associated research fields include cysteine lipid acylation in the proteome, endosome-to-TGN trafficking and rAAV transduction, and post-resection homologous recombination DNA repair.
| Species | Application | Title |
|---|---|---|
J Biol Chem Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome | ||
bioRxiv TBC1D23-AAVR Interaction Drives Endosome-to-TGN Trafficking Required for rAAV Transduction. | ||
Reviews
What customers say
Users report positive experiences with this antibody for Western Blot. One reviewer rated it 5 stars, describing it as "very good for WB." Another rated it 4 stars when used on mouse heart tissue, noting that it produced a double band with the upper band being much denser than the lower band. Overall, the antibody is well-received for WB applications, with users finding it effective, though some may observe multiple bands depending on the tissue used.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (01-31-2025) | very good for WB
|
Wei (Verified Customer) (06-28-2020) | Double band for whole heart tissues. The upper band is much dense than the lower band.
|
