STX5 Polyclonal antibody

STX5 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 26711-1-AP

Synonyms

SED5, STX5, STX5A, syntaxin 5

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Validation Data Gallery

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  WB analysis using 26711-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26711-1-AP (STX5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 26711-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26711-1-AP (STX5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26711-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26711-1-AP (STX5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26711-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26711-1-AP (STX5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26711-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26711-1-AP (STX5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 26711-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 26711-1-AP (STX5 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 26711-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 26711-1-AP (STX5 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, HepG2 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

26711-1-AP targets STX5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STX5 fusion protein Ag25098
• Full Name: syntaxin 5
• Calculated Molecular Weight: 32 kDa, 34 kDa, 42 kDa
• Observed Molecular Weight: 35 and 42 kDa
• GenBank Accession Number: BC012137
• Gene Symbol: STX5
• Gene ID (NCBI): 6811
• RRID: AB_2880609
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Biol Chem; Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome; Authors - Hana Nůsková

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wei (Verified Customer) (06-28-2020)

Double band for whole heart tissues. The upper band is much dense than the lower band.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart