Syntaxin 7 Polyclonal antibody

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  WB analysis of MDCK cells using 12322-1-AP

  siRNA result of 12322-1-AP (anti-STX7) by Dr.Kazunari Yamashita.

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  WB analysis of mouse spleen using 12322-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 12322-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12322-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 12322-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12322-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 12322-1-AP

  IP result of anti-Syntaxin 7 (IP:12322-1-AP, 3ug; Detection:12322-1-AP 1:800) with mouse heart tissue lysate 4000ug.

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  IHC staining of human medulloblastoma using 12322-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma tissue slide using 12322-1-AP (Syntaxin 7 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human medulloblastoma using 12322-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma tissue slide using 12322-1-AP (Syntaxin 7 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 12322-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using Syntaxin 7 antibody (12322-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

Syntaxin 7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12322-1-AP

Publications(12)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Syntaxin-7, STX7

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Tested Applications

• Positive WB detected in: mouse spleen tissue, MDCK cells
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human medulloblastoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 2 publications below

Product Information

12322-1-AP targets Syntaxin 7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, canine samples.

• Tested Reactivity: human, mouse, canine
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2975

  Product name: Recombinant human STX7 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-244 aa of BC011975

  Sequence: MSYTPGVGGDPAQLAQRISSNIQKITQCSVEIQRTLNQLGTPQDSPELRQQLQQKQQYTNQLAKETDKYIKEFGSLPTTPSEQRQRKIQKDRLVAEFTTSLTNFQKVQRQAAEREKEFVARVRASSRVSGSFPEDSSKERNLVSWESQTQPQVQVQDEEITEDDLRLIHERESSIRQLEADIMDINEIFKDLGMMIHEQGDVIDSIEANVENAEVHVQQANQQLSRAADYQRKSRKTLCIIILI

• Full Name: syntaxin 7
• Calculated Molecular Weight: 261 aa, 30 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC011975
• Gene Symbol: Syntaxin 7
• Gene ID (NCBI): 8417
• RRID: AB_2239979
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15400
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Proc Natl Acad Sci U S A; Crucial role of the hydrophobic pocket region of Munc18 protein in mast cell degranulation.; Authors - Bin Na-Ryum NR
rat	WB,CoIP	J Cell Biol; VAMP4 regulates insulin levels by targeting secretory granules to lysosomes; Authors - Min Li ; KD Validated
human	WB	Biochem J; Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.; Authors - Nazma Malik
human	WB	Cell Logist; Interaction with the effector dynamin-related protein 1 (Drp1) is an ancient function of Rab32 subfamily proteins.; Authors - Carolina G Ortiz-Sandoval
human,mouse	WB,IF	Neurobiol Dis; Syntaxin 7 modulates seizure activity in epilepsy; Authors - Junhong Wu
human	WB	J Biol Chem; Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome; Authors - Hana Nůsková

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lianne (Verified Customer) (09-24-2025)

50ug RAW 264.7 mouse cell lysate; proteins resolved in 12% SDS-PAGE gel, transferred onto PVDF. Probed with 1:500 dilution of anti-Syntaxin 7 antibody overnight at 4oC. Detected using IRDye 800CW anti-rabbit IgG.

• Applications: Western Blot
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: RAW 264.7 mouse macrophage cell line

Syntaxin 7 Antibody Western Blot validation (1/500 dilution) in RAW 264.7 mouse macrophage cell line (Cat no:12322-1-AP)

FH

Olga (Verified Customer) (01-17-2025)

Works perfectly in Western blot. Recognizes both human and mouse endogenous Stx 7 . Gives a single clear band in most tissue and cell lysates tested.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T, Neuro2A, mouse primary cerebellar neurons, mouse tissues (heart, liver, lung, forebrain)

FH

Christian (Verified Customer) (10-12-2020)

The signal is strong and clean

• Applications: Western Blot
• Cell Tissue Type: b16f10