Syntaxin 7 Polyclonal antibody

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  WB analysis of MDCK cells using 12322-1-AP

  siRNA result of 12322-1-AP (anti-STX7) by Dr.Kazunari Yamashita.

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  WB analysis of mouse spleen using 12322-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 12322-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12322-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12322-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 12322-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 12322-1-AP

  IP result of anti-Syntaxin 7 (IP:12322-1-AP, 3ug; Detection:12322-1-AP 1:800) with mouse heart tissue lysate 4000ug.

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  IHC staining of human medulloblastoma using 12322-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma tissue slide using 12322-1-AP (Syntaxin 7 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human medulloblastoma using 12322-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma tissue slide using 12322-1-AP (Syntaxin 7 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 12322-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using Syntaxin 7 antibody (12322-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

Syntaxin 7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12322-1-AP

Publications(13)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Syntaxin-7, STX7

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Tested Applications

• Positive WB detected in: mouse spleen tissue, MDCK cells
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human medulloblastoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 2 publications below

Product Information

12322-1-AP targets Syntaxin 7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, canine samples.

• Tested Reactivity: human, mouse, canine
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2975

  Product name: Recombinant human STX7 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-244 aa of BC011975

  Sequence: MSYTPGVGGDPAQLAQRISSNIQKITQCSVEIQRTLNQLGTPQDSPELRQQLQQKQQYTNQLAKETDKYIKEFGSLPTTPSEQRQRKIQKDRLVAEFTTSLTNFQKVQRQAAEREKEFVARVRASSRVSGSFPEDSSKERNLVSWESQTQPQVQVQDEEITEDDLRLIHERESSIRQLEADIMDINEIFKDLGMMIHEQGDVIDSIEANVENAEVHVQQANQQLSRAADYQRKSRKTLCIIILI

• Full Name: syntaxin 7
• Calculated Molecular Weight: 261 aa, 30 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC011975
• Gene Symbol: Syntaxin 7
• Gene ID (NCBI): 8417
• RRID: AB_2239979
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15400
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin 7() is a SNARE protein localized to late endosomes and lysosomes, mediating vesicle fusion and intracellular trafficking. It plays essential roles in autophagy, pathogen degradation, and cholesterol transport through interactions with VAMP8 and Vti1b.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Proc Natl Acad Sci U S A; Crucial role of the hydrophobic pocket region of Munc18 protein in mast cell degranulation.; Authors - Bin Na-Ryum NR
rat	WB,CoIP	J Cell Biol; VAMP4 regulates insulin levels by targeting secretory granules to lysosomes; Authors - Min Li ; KD Validated
human	WB	Biochem J; Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.; Authors - Nazma Malik
human	WB	Cell Logist; Interaction with the effector dynamin-related protein 1 (Drp1) is an ancient function of Rab32 subfamily proteins.; Authors - Carolina G Ortiz-Sandoval
mouse	CoIP	Front Cell Dev Biol; Inceptor facilitates acrosomal vesicle formation in spermatids and is required for male fertility; Authors - Sara Bilekova
		PLoS Pathog; Manganese is a potent inducer of lysosomal activity that inhibits de novo HBV infection; Authors - Lin Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lianne (Verified Customer) (09-24-2025)

50ug RAW 264.7 mouse cell lysate; proteins resolved in 12% SDS-PAGE gel, transferred onto PVDF. Probed with 1:500 dilution of anti-Syntaxin 7 antibody overnight at 4oC. Detected using IRDye 800CW anti-rabbit IgG.

• Applications: Western Blot
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: RAW 264.7 mouse macrophage cell line

Syntaxin 7 Antibody Western Blot validation (1/500 dilution) in RAW 264.7 mouse macrophage cell line (Cat no:12322-1-AP)

FH

Olga (Verified Customer) (01-17-2025)

Works perfectly in Western blot. Recognizes both human and mouse endogenous Stx 7 . Gives a single clear band in most tissue and cell lysates tested.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T, Neuro2A, mouse primary cerebellar neurons, mouse tissues (heart, liver, lung, forebrain)

FH

Christian (Verified Customer) (10-12-2020)

The signal is strong and clean

• Applications: Western Blot
• Cell Tissue Type: b16f10