Tested Applications
| Positive WB detected in | mouse spleen tissue, MDCK cells, rat spleen tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human medulloblastoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12322-1-AP targets Syntaxin 7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, canine samples.
| Tested Reactivity | human, mouse, canine |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2975 Product name: Recombinant human STX7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-244 aa of BC011975 Sequence: MSYTPGVGGDPAQLAQRISSNIQKITQCSVEIQRTLNQLGTPQDSPELRQQLQQKQQYTNQLAKETDKYIKEFGSLPTTPSEQRQRKIQKDRLVAEFTTSLTNFQKVQRQAAEREKEFVARVRASSRVSGSFPEDSSKERNLVSWESQTQPQVQVQDEEITEDDLRLIHERESSIRQLEADIMDINEIFKDLGMMIHEQGDVIDSIEANVENAEVHVQQANQQLSRAADYQRKSRKTLCIIILI Predict reactive species |
| Full Name | syntaxin 7 |
| Calculated Molecular Weight | 261 aa, 30 kDa |
| Observed Molecular Weight | 30-35 kDa |
| GenBank Accession Number | BC011975 |
| Gene Symbol | Syntaxin 7 |
| Gene ID (NCBI) | 8417 |
| RRID | AB_2239979 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15400 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Syntaxin 7(STX7) is a SNARE protein localized to late endosomes and lysosomes, mediating vesicle fusion and intracellular trafficking. It plays essential roles in autophagy, pathogen degradation, and cholesterol transport through interactions with VAMP8 and Vti1b.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Syntaxin 7 antibody 12322-1-AP | Download protocol |
| IHC protocol for Syntaxin 7 antibody 12322-1-AP | Download protocol |
| IP protocol for Syntaxin 7 antibody 12322-1-AP | Download protocol |
| WB protocol for Syntaxin 7 antibody 12322-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-Syntaxin 7 antibody (Cat# 12322-1-AP) has 13 citations published in journals including PNAS, Cell Reports, Journal of Cell Biology, Journal of Biological Chemistry, FASEB Journal, Diabetes, Neurobiology of Disease, PLoS Pathogens, Frontiers in Cell and Developmental Biology, Biomedicines, Biochemical Journal, and Cell Logistics. Research fields span mast cell degranulation, insulin secretion, autophagosome-lysosome fusion, epilepsy, spermatogenesis, lysosomal activity, ovarian cancer immunotherapy, neuroinflammation, phosphoproteomics, and Rab32–Drp1 interactions.
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Crucial role of the hydrophobic pocket region of Munc18 protein in mast cell degranulation. | ||
Diabetes Optimised proteomic analysis of insulin granules from MIN6 cells identifies Scamp3, a novel regulator of insulin secretion and content | ||
Cell Rep Human YKT6 forms priming complex with STX17 and SNAP29 to facilitate autophagosome-lysosome fusion | ||
J Cell Biol VAMP4 regulates insulin levels by targeting secretory granules to lysosomes
| ||
Front Cell Dev Biol Inceptor facilitates acrosomal vesicle formation in spermatids and is required for male fertility |
Reviews
What customers say
All three reviews rated this anti-Syntaxin 7 antibody 5 stars for Western Blot. Users tested it on a range of samples including RAW 264.7, HEK293T, Neuro2A, mouse primary cerebellar neurons, mouse tissues (heart, liver, lung, forebrain), and b16f10 cells, at dilutions of 1:500 to 1:1000. The antibody consistently produced a single, strong, and clean band, with confirmed recognition of both human and mouse endogenous Stx7. Overall, reviewers described the performance as reliable and excellent across species and sample types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lianne (Verified Customer) (09-24-2025) | 50ug RAW 264.7 mouse cell lysate; proteins resolved in 12% SDS-PAGE gel, transferred onto PVDF. Probed with 1:500 dilution of anti-Syntaxin 7 antibody overnight at 4oC. Detected using IRDye 800CW anti-rabbit IgG.
 |
Olga (Verified Customer) (01-17-2025) | Works perfectly in Western blot. Recognizes both human and mouse endogenous Stx 7 . Gives a single clear band in most tissue and cell lysates tested.
|
Christian (Verified Customer) (10-12-2020) | The signal is strong and clean
|
