STXBP2 Monoclonal antibody, PBS Only

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  WB analysis using 66238-1-Ig (same clone as 66238-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66238-1-Ig (STXBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66238-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66238-1-Ig (same clone as 66238-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66238-1-Ig (STXBP2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66238-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 66238-1-Ig (same clone as 66238-1-PBS)

  IP result of anti-STXBP2 (IP:66238-1-Ig, 5ug; Detection:66238-1-Ig 1:2000) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 66238-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66238-1-Ig (same clone as 66238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66238-1-Ig (STXBP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66238-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66238-1-Ig (same clone as 66238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66238-1-Ig (STXBP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66238-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66238-1-Ig (same clone as 66238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66238-1-Ig (STXBP2 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66238-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66238-1-Ig (same clone as 66238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66238-1-Ig (STXBP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66238-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66238-1-Ig (same clone as 66238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66238-1-Ig (STXBP2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66238-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66238-1-Ig (same clone as 66238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66238-1-Ig (STXBP2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66238-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66238-1-Ig (same clone as 66238-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using STXBP2 antibody (66238-1-Ig, Clone: 3G3A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66238-1-PBS in a different storage buffer formulation.

STXBP2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 66238-1-PBS

Clone No.3G3A12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

3G3A12, Hunc18b, MUNC18 2, Munc18-2, pp10122

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Product Information

66238-1-PBS targets STXBP2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7640

  Product name: Recombinant human STXBP2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 241-590 aa of BC002869

  Sequence: LHELTFQAMAYDLLDIEQDTYRYETTGLSEAREKAVLLDEDDDLWVELRHMHIADVSKKVTELLRTFCESKRLTTDKANIKDLSQILKKMPQYQKELNKYSTHLHLADDCMKHFKGSVEKLCSVEQDLAMGSDAEGEKIKDSMKLIVPVLLDAAVPAYDKIRVLLLYILLRNGVSEENLAKLIQHANVQAHSSLIRNLEQLGGTVTNPGGSGTSSRLEPRERMEPTYQLSRWTPVIKDVMEDAVEDRLDRNLWPFVSDPAPTASSQAAVSARFGHWHKNKAGIEARAGPRLIVYVMGGVAMSEMRAAYEVTRATEGKWEVLIGSSHILTPTRFLDDLKALDKKLEDIALP

• Full Name: syntaxin binding protein 2
• Calculated Molecular Weight: 593 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC002869
• Gene Symbol: STXBP2
• Gene ID (NCBI): 6813
• RRID: AB_2881627
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q15833
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Syntaxin-binding protein 2 (STXBP2, also known as Unc18-2) belongs to the STXBP/unc-18/SEC1 family. STXBP2 is involved in intracellular trafficking, control of SNARE (soluble NSF attachment protein receptor) complex assembly, and the release of cytotoxic granules by natural killer cells. Mutations in the STXBP2 gene are associated with familial hemophagocytic lymphohistiocytosis.