STXBP3 Polyclonal antibody

STXBP3 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13764-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

MUNC18 3, MUNC18C, Platelet Sec1 protein, Protein unc 18 homolog 3, Protein unc 18 homolog C

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIPIHC

WB analysis of HepG2 using 13764-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 13764-1-AP (STXBP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 13764-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 13764-1-AP (STXBP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 13764-1-AP
IP result of anti-STXBP3 (IP:13764-1-AP, 4ug; Detection:13764-1-AP 1:500) with K-562 cells lysate 1200ug.
IHC staining of human lymphoma using 13764-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma using 13764-1-AP (STXBP3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lymphoma using 13764-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma using 13764-1-AP (STXBP3 antibody) at dilution of 1:100 (under 40x lens).

Positive WB detected in	HepG2 cells, K-562 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

13764-1-AP targets STXBP3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4747 Product name: Recombinant human STXBP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 242-592 aa of BC038099 Sequence: FDPVSTVLHELTFQAMAYDLLPIENDTYKYKTDGKEKEAILEEEDDLWVRIRHRHIAVVLEEIPKLMKEISSTKKATEGKTSLSALTQLMKKMPHFRKQITKQVVHLNLAEDCMNKFKLNIEKLCKTEQDLALGTDAEGQKVKDSMRVLLPVLLNKNHDNCDKIRAILLYIFSINGTTEENLDRLIQNVKIENESDMIRNWSYLGVPIVPQSQQGKPLRKDRSAEETFQLSRWTPFIKDIMEDAIDNRLDSKEWPYCSQCPAVWNGSGAVSARQKPRANYLEDRKNGSKLIVFVIGGITYSEVRCAYEVSQAHKSCEVIIGSTHVLTPKKLLDDIKMLNKPKDKVSLIKDE Predict reactive species
Full Name	syntaxin binding protein 3
Calculated Molecular Weight	592 aa, 68 kDa
Observed Molecular Weight	68 kDa
GenBank Accession Number	BC038099
Gene Symbol	STXBP3
Gene ID (NCBI)	6814
RRID	AB_2196832
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O00186
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IHC protocol for STXBP3 antibody 13764-1-AP	Download protocol
IP protocol for STXBP3 antibody 13764-1-AP	Download protocol
WB protocol for STXBP3 antibody 13764-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB	Diabetes PMID: 20460426 Authors - Pontus Boström View Article
mouse	WB	J Biol Chem microRNA-15b contributes to depression-like behavior in mice by affecting synaptic protein levels and function in the nucleus accumbens. Authors - Li Guo View Article
rat	WB	Neuroscience SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain. Authors - Xing-Lian Duan View Article