STXBP4 Polyclonal antibody

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  WB analysis using 14231-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14231-1-AP (STXBP4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14231-1-AP

  PC-3 lysates were subjected to SDS PAGE followed by western blot with 14231-1-AP (STXBP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 14231-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14231-1-AP (STXBP4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 14231-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14231-1-AP (STXBP4 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of BxPC-3 using 14231-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using STXBP4 antibody (14231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

STXBP4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14231-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

STX4-interacting protein, Synip, Syntaxin 4-interacting protein, Syntaxin-binding protein 4

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Tested Applications

• Positive WB detected in: HepG2 cells, PC-3 cells, HuH-7 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14231-1-AP targets STXBP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5471

  Product name: Recombinant human STXBP4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-245 aa of BC041485

  Sequence: MIGVSFEEAKSIITGAKLSTRLESAWEIAFIRQKSDNIQPENLSCTSLIEASGEYGPQASTLSLFSSPPEILIPKTSSTPKTNNDILSSCEIKTGYNKTVQIPITSENSTVGLSNTDVASAWTENYGLQEKISLNPSVRFKAEKLEMALNYLGIQPTKEQHQALRQQVQADSKGTVSFGDFVQVARNLFCLQLDEVNVGAHEISNILDSQLLPCDSSEADEMERLKCERDDALKEVNTLKWPGQN

• Full Name: syntaxin binding protein 4
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC041485
• Gene Symbol: STXBP4
• Gene ID (NCBI): 252983
• RRID: AB_2196980
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6ZWJ1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STXBP4, also known as Synip, plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane. STXBP4 inhibits the translocation of GLUT4 from intracellular vesicles to the plasma membrane by STX4A binding and preventing the interaction between STX4A and VAMP2(PMID: 10394363).