STYK1 Polyclonal antibody

STYK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 18028-1-AP

Synonyms

NOK, Protein PK unique, STYK1, SuRTK106, Tyrosine protein kinase STYK1

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Validation Data Gallery

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  WB analysis of human brain using 18028-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18028-1-AP (STYK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18028-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18028-1-AP (STYK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 18028-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18028-1-AP (STYK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18028-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18028-1-AP (STYK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human brain tissue, HeLa cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

18028-1-AP targets STYK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STYK1 fusion protein Ag12594
• Full Name: serine/threonine/tyrosine kinase 1
• Calculated Molecular Weight: 422 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC093822
• Gene Symbol: STYK1
• Gene ID (NCBI): 55359
• RRID: AB_10603477
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Oncol Lett; Downregulation of GLUT3 impairs STYK1/NOK-mediated metabolic reprogramming and proliferation in NIH-3T3 cells.; Authors - Weiye Shi
human	WB	Heliyon; STYK1/NOK affects cell cycle late mitosis and directly interacts with anaphase-promoting complex activator CDH1; Authors - Su-Lin Zeng
mouse	WB	Biochem Biophys Res Commun; Induction of chronic lymphocytic leukemia-like disease in STYK1/NOK transgenic mice; Authors - Yin Yang