|Positive WB detected in||HEK-293 cells, L02 cells, SH-SY5Y cells, HepG2 cells, PC-3 cells, mouse brain tissue, mouse liver tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human colon cancer tissue, human heart tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12627-1-AP targets SUCLA2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SUCLA2 fusion protein Ag3319|
|Full Name||succinate-CoA ligase, ADP-forming, beta subunit|
|Calculated molecular weight||463 aa, 50 kDa|
|Observed molecular weight||48-50 kDa|
|GenBank accession number||BC027587|
|Gene ID (NCBI)||8803|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SUCLA2(succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial) is also named as SCS-betaA and belongs to the succinate/malate CoA ligase beta subunit family. It can catalyzes the ATP-dependent ligation of succinate and CoA to form succinyl-CoA.Defects in SUCLA2 are the cause of mitochondrial DNA depletion syndrome type 5 (MTDPS5).
|Product Specific Protocols|
|WB protocol for SUCLA2 antibody 12627-1-AP||Download protocol|
|IHC protocol for SUCLA2 antibody 12627-1-AP||Download protocol|
|IF protocol for SUCLA2 antibody 12627-1-AP||Download protocol|
|IP protocol for SUCLA2 antibody 12627-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.
2,2',4,4'-Tetrabromodiphenyl ether disrupts spermatogenesis, impairs mitochondrial function and induces apoptosis of early leptotene spermatocytes in rats.
J Bioenerg Biomembr
Localization of SUCLA2 and SUCLG2 subunits of succinyl CoA ligase within the cerebral cortex suggests the absence of matrix substrate-level phosphorylation in glial cells of the human brain.
J Inherit Metab Dis
Succinate-CoA ligase deficiency due to mutations in SUCLA2 and SUCLG1: phenotype and genotype correlations in 71 patients.
Two transgenic mouse models for beta subunit components of succinate-CoA ligase yielding pleiotropic metabolic alterations.
Brain Struct Funct
Exclusive neuronal detection of KGDHC-specific subunits in the adult human brain cortex despite pancellular protein lysine succinylation.