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  • KD/KO Validated

SUCLA2 Polyclonal antibody

SUCLA2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12627-1-AP

Synonyms

A BETA, SCS betaA, SUCLA2



Tested Applications

Positive WB detected inHEK-293 cells, L02 cells, SH-SY5Y cells, HepG2 cells, PC-3 cells, mouse brain tissue, mouse liver tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12627-1-AP targets SUCLA2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUCLA2 fusion protein Ag3319
Full Name succinate-CoA ligase, ADP-forming, beta subunit
Calculated molecular weight 463 aa, 50 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberBC027587
Gene symbol SUCLA2
Gene ID (NCBI) 8803
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SUCLA2(succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial) is also named as SCS-betaA and belongs to the succinate/malate CoA ligase beta subunit family. It can catalyzes the ATP-dependent ligation of succinate and CoA to form succinyl-CoA.Defects in SUCLA2 are the cause of mitochondrial DNA depletion syndrome type 5 (MTDPS5).

Protocols

Product Specific Protocols
WB protocol for SUCLA2 antibody 12627-1-APDownload protocol
IHC protocol for SUCLA2 antibody 12627-1-APDownload protocol
IF protocol for SUCLA2 antibody 12627-1-APDownload protocol
IP protocol for SUCLA2 antibody 12627-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation

Authors - Sophie Trefely
humanWB

Nat Commun

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Authors - Xiao-Hui Wang
humanWB,IHC

Oncogene

Pharmacologically targetable vulnerability in prostate cancer carrying RB1-SUCLA2 deletion.

Authors - Susumu Kohno
  • KO Validated
human,mouseWB,IHC

Oncogene

MUC1 triggers lineage plasticity of Her2 positive mammary tumors

Authors - Zhi Pang
humanIF

Brain Struct Funct

Exclusive neuronal expression of SUCLA2 in the human brain.

Authors - Dobolyi Arpád A
ratIHC

Front Cell Neurosci

Investigations into photoreceptor energy metabolism during experimental retinal detachment

Authors - Glyn Chidlow