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  • KD/KO Validated

SUCLA2 Polyclonal antibody

SUCLA2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12627-1-AP

Synonyms

A BETA, SCS betaA, SUCLA2



Tested Applications

Positive WB detected inHEK-293 cells, L02 cells, SH-SY5Y cells, HepG2 cells, PC-3 cells, mouse brain tissue, mouse liver tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12627-1-AP targets SUCLA2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUCLA2 fusion protein Ag3319
Full Name succinate-CoA ligase, ADP-forming, beta subunit
Calculated molecular weight 463 aa, 50 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberBC027587
Gene symbol SUCLA2
Gene ID (NCBI) 8803
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SUCLA2(succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial) is also named as SCS-betaA and belongs to the succinate/malate CoA ligase beta subunit family. It can catalyzes the ATP-dependent ligation of succinate and CoA to form succinyl-CoA.Defects in SUCLA2 are the cause of mitochondrial DNA depletion syndrome type 5 (MTDPS5).

Protocols

Product Specific Protocols
WB protocol for SUCLA2 antibody 12627-1-APDownload protocol
IHC protocol for SUCLA2 antibody 12627-1-APDownload protocol
IF protocol for SUCLA2 antibody 12627-1-APDownload protocol
IP protocol for SUCLA2 antibody 12627-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Haematologica

Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.

Authors - Nunziata Maio
ratWB

Reprod Toxicol

2,2',4,4'-Tetrabromodiphenyl ether disrupts spermatogenesis, impairs mitochondrial function and induces apoptosis of early leptotene spermatocytes in rats.

Authors - Shaoping Huang
IF

J Bioenerg Biomembr

Localization of SUCLA2 and SUCLG2 subunits of succinyl CoA ligase within the cerebral cortex suggests the absence of matrix substrate-level phosphorylation in glial cells of the human brain.

Authors - Arpád Dobolyi
humanWB

J Inherit Metab Dis

Succinate-CoA ligase deficiency due to mutations in SUCLA2 and SUCLG1: phenotype and genotype correlations in 71 patients.

Authors - Rosalba Carrozzo
mouseWB

Biochem J

Two transgenic mouse models for beta subunit components of succinate-CoA ligase yielding pleiotropic metabolic alterations.

Authors - Gergely Kacso
  • KO Validated
humanWB

Brain Struct Funct

Exclusive neuronal detection of KGDHC-specific subunits in the adult human brain cortex despite pancellular protein lysine succinylation.

Authors - Arpad Dobolyi