Validation Data Gallery
|Positive WB detected in
|HEK-293 cells, L02 cells, SH-SY5Y cells, HepG2 cells, PC-3 cells, mouse brain tissue, mouse liver tissue
|Positive IP detected in
|Positive IHC detected in
|human colon cancer tissue, human heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:1000-1:6000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
12627-1-AP targets SUCLA2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|SUCLA2 fusion protein Ag3319
|succinate-CoA ligase, ADP-forming, beta subunit
|Calculated molecular weight
|463 aa, 50 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
SUCLA2(succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial) is also named as SCS-betaA and belongs to the succinate/malate CoA ligase beta subunit family. It can catalyzes the ATP-dependent ligation of succinate and CoA to form succinyl-CoA.Defects in SUCLA2 are the cause of mitochondrial DNA depletion syndrome type 5 (MTDPS5).
|Product Specific Protocols
|WB protocol for SUCLA2 antibody 12627-1-AP
|IHC protocol for SUCLA2 antibody 12627-1-AP
|IF protocol for SUCLA2 antibody 12627-1-AP
|IP protocol for SUCLA2 antibody 12627-1-AP
|Click here to view our Standard Protocols
Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation
Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.
Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging
Pharmacologically targetable vulnerability in prostate cancer carrying RB1-SUCLA2 deletion.
MUC1 triggers lineage plasticity of Her2 positive mammary tumors
Brain Struct Funct
Exclusive neuronal expression of SUCLA2 in the human brain.