SULF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SULF2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12260-1-AP

Synonyms

Extracellular sulfatase Sulf 2, HSULF 2, KIAA1247, SULF2, sulfatase 2

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Validation Data Gallery

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  WB analysis of mouse liver using 12260-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12260-1-AP (SULF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 12260-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12260-1-AP (SULF2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 12260-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12260-1-AP (SULF2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 12260-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 12260-1-AP (SULF2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 12260-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 12260-1-AP (SULF2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: human breast cancer tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

12260-1-AP targets SULF2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SULF2 fusion protein Ag2915
• Full Name: sulfatase 2
• Calculated Molecular Weight: 870 aa, 96 kDa
• Observed Molecular Weight: 50 kDa, 130-150 kDa
• GenBank Accession Number: BC020962
• Gene Symbol: SULF2
• Gene ID (NCBI): 55959
• RRID: AB_11183762
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SULF2, also named as KIAA1247, belongs to the sulfatase family. It exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. SULF2 can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. SULF2 has several forms: the unglycosylated 100 kDa SULF2, the glycosylated 120-150 kDa SULF2, the cleaved 72-75 kDa and 50 kDa SULF2.

Publications

Species	Application	Title
mouse		J Exp Med; CXCR4 regulates Plasmodium development in mouse and human hepatocytes.; Authors - Hironori Bando
human,mouse	WB,IHC,IF	Cancer Lett; SULF2 enhances GDF15-SMAD axis to facilitate the initiation and progression of pancreatic cancer.; Authors - Ruizhe He ; KO Validated