SULT1A3 Polyclonal antibody
SULT1A3 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 19499-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HT-29 cells, HepG2 cells, HuH-7 cells, Caco-2 cells, MCF-7 cells, mouse liver tissue, COLO 320 cells |
Positive IP detected in | COLO 320 cells |
Positive IHC detected in | human hepatocirrhosis tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
IHC | See 1 publications below |
Product Information
19499-1-AP targets SULT1A3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | sulfotransferase family, cytosolic, 1A, phenol-preferring, member 3 |
Calculated Molecular Weight | 34 kDa |
Observed Molecular Weight | 30-34 kDa |
GenBank Accession Number | NM_177552 |
Gene Symbol | SULT1A3 |
Gene ID (NCBI) | 6818 |
RRID | AB_10642560 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SULT1A3, also named as STM and SULT1A4, belongs to the sulfotransferase 1 family. SULT1A3 catalyzes the sulfate conjugation of phenolic monoamines (neurotransmitters such as dopamine, norepinephrine and serotonin) and phenolic and catechol drugs.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for SULT1A3 antibody 19499-1-AP | Download protocol |
IHC protocol for SULT1A3 antibody 19499-1-AP | Download protocol |
IP protocol for SULT1A3 antibody 19499-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancer Lett Expression of sulfotransferases and sulfatases in human breast cancer: impact on resveratrol metabolism. | ||
J Adv Res Endogenous hydrogen sulfide improves vascular remodeling through PPARδ/SOCS3 signaling. |