SULT1E1 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• SULT1E1 Polyclonal antibody (12522-1-AP)

• Featured Product
• KD/KO Validated

SULT1E1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12522-1-AP Copied!

Publications (22)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

EST, EST 1, Estrogen sulfotransferase, ST1E1, STE, Sulfotransferase 1E1, SULT1E1

• 

  WB analysis of human lung using 12522-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 12522-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human adrenal gland using 12522-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 12522-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human kidney using 12522-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse liver using 12522-1-AP

  IP result of anti-SULT1E1 (IP:12522-1-AP, 4ug; Detection:12522-1-AP 1:400) with mouse liver tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 12522-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 12522-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for SULT1E1 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: human lung tissue, human adrenal gland tissue, human kidney tissue, human liver tissue, mouse kidney tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12522-1-AP targets SULT1E1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3216

  Product name: Recombinant human SULT1E1 protein

  Source: e coli.-derived, PKG

  Tag: GST

  Domain: 1-294 aa of BC027956

  Sequence: MNSELDYYEKFEEVHGILMYKDFVKYWDNVEAFQARPDDLVIATYPKSGTTWVSEIVYMIYKEGDVEKCKEDVIFNRIPFLECRKENLMNGVKQLDEMNSPRIVKTHLPPELLPASFWEKDCKIIYLCRNAKDVAVSFYYFFLMVAGHPNPGSLPEFVEKFMQGQVPYGSWYKHVKSWWEKGKSPRVLFLFYEDLKEDIRKEVIKLIHFLERKPSEELVDRIIHHTSFQEMKNNPSTNYTTLPDEIMNQKLSPFMRKGITGDWKNHFTVALNEKFDKHYEQQMKESTLKFRTEI

• Full Name: sulfotransferase family 1E, estrogen-preferring, member 1
• Calculated Molecular Weight: 294 aa, 35 kDa
• Observed Molecular Weight: 32-35 kDa
• GenBank Accession Number: BC027956
• Gene Symbol: SULT1E1
• Gene ID (NCBI): 6783
• RRID: AB_2302772
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49888
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SULT1E1, also known as estrogen sulfotransferase, catalyzes the sulfation of endogenous estrogens as well as xenobiotic estrogen-like chemicals, converting them into the inactive form. SULT1E1 is a 33-35 kDa cytosolic protein that has been detected in human hepatic and nonhepatic tissues (17035602). SULT1E1 is expressed in breast and endometrial tissues, and some studies have investigated its implication in tumor development. Higher expression of SULT1E1 was observed in breast cancer tissues compared with normal breast tissues (22380844). This antibody detected a major band around 35 kD in lysates of mouse kidney, human liver and so on.

Publications

What published studies show

The SULT1E1 (Estrogen Sulfotransferase) antibody (Cat# 12522-1-AP) has 22 citations across journals including Nature Communications, J Am Soc Nephrol, J Biol Chem, Endocrinology, Biochemical Pharmacology, Scientific Reports, Cancer Science, and Drug Metabolism and Disposition. Research fields span estrogen metabolism, breast cancer, hepatocellular carcinoma, acute kidney injury, sepsis, COPD, lung adenocarcinoma, insulin sensitivity, lipid metabolism, and drug metabolism.

Automatically generated from the 22 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nat Commun; Oestrogen sulfotransferase ablation sensitizes mice to sepsis.; Authors - Xiaojuan Chai
human	WB,IF	J Adv Res; Naringenin in Si-Ni-San formula inhibits chronic psychological stress-induced breast cancer growth and metastasis by modulating estrogen metabolism through FXR/EST pathway.; Authors - Juping Zhang
mouse	IF,WB	J Nanobiotechnology; Developing liver-targeted naringenin nanoparticles for breast cancer endocrine therapy by promoting estrogen metabolism; Authors - Yuying Zhao
	IHC	J Am Soc Nephrol; Inhibition of Estrogen Sulfotransferase (SULT1E1/EST) Ameliorates Ischemic Acute Kidney Injury in Mice.; Authors - Anne C Silva Barbosa
human	WB	Biochem Pharmacol; PXR phosphorylated at Ser350 transduces a glucose signal to repress the estrogen sulfotransferase gene in human liver cells and fasting signal in mouse livers.; Authors - Hao Hu
human	WB	Biochem Pharmacol; Farnesoid X receptor regulates SULT1E1 expression through inhibition of PGC1α binding to HNF4α.; Authors - Shuai Wang