SULT2A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SULT2A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16348-1-AP

Synonyms

ST2, ST2A1, EC:2.8.2.2, EC:2.8.2.14, DHEA-ST8

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Validation Data Gallery

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  WB analysis of human adrenal gland using 16348-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 16348-1-AP (SULT2A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 16348-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16348-1-AP (SULT2A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16348-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16348-1-AP (SULT2A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 16348-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16348-1-AP (SULT2A1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16348-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16348-1-AP (SULT2A1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human adrenal gland tissue, human liver tissue, L02 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

16348-1-AP targets SULT2A1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SULT2A1 fusion protein Ag9542
• Full Name: sulfotransferase family, cytosolic, 2A, dehydroepiandrosterone (DHEA)-preferring, member 1
• Calculated Molecular Weight: 285 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC020755
• Gene Symbol: SULT2A1
• Gene ID (NCBI): 6822
• RRID: AB_2197770
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Apoptosis; An integrated investigation of sulfotransferases (SULTs) in hepatocellular carcinoma and identification of the role of SULT2A1 on stemness; Authors - Hao Peng ; KD Validated
rat		Chem Biol Interact; Ginsenoside Rg1 alleviates ANIT-induced intrahepatic cholestasis in rats via activating farnesoid X receptor and regulating transporters and metabolic enzymes.; Authors - Qing Xiao
human	WB	Drug Metab Dispos; 17β-Estradiol (E2) Induces Sulfotransferase (SULT) 2A1 Expression through Estrogen Receptor (ER) α; Authors - Wei Li
rat	WB	Naunyn Schmiedebergs Arch Pharmacol; Molecular mechanism of geniposide against ANIT-induced intrahepatic cholestasis by integrative analysis of transcriptomics and metabolomics; Authors - Junyi Zhang