SUMO1 Recombinant Matched Antibody Pair, PBS Only

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• SUMO1 Recombinant Matched Antibody Pair, PBS Only (MP03279-2)

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.195-12.5 ng/mL

Cat no : MP03279-2

Synonyms

GAP-modifying protein 1, GMP1, OK/SW-cl.43, Sentrin, Small ubiquitin related modifier 1

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP03279-2

  Sandwich ELISA standard curve of MP03279-2, Human SUMO1 Recombinant Matched Antibody Pair - PBS only. 87942-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0414. 87942-2-PBS was HRP conjugated as the detection antibody. Range: 0.195-12.5 ng/mL.

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  Sandwich ELISA sample test of MP03279-2

  The mean SUMO1 concentration was determined to be 19.73 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load, 33.97 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load and 21.00 ng/mL in NIH/3T3 cell extract based on a 1.50 mg/mL extract load.

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Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP03279-2 targets SUMO1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.195-12.5 ng/mL (Sandwich ELISA);

Capture antibody: SUMO1 Recombinant monoclonal antibody, PBS Only (Capture) 87942-4-PBS (253578D11). 100 μg. Concentration 1 mg/ml.

Detection antibody: SUMO1 Recombinant monoclonal antibody, PBS Only (Detector) 87942-2-PBS (253578D5). 100 μg. Concentration 1 mg/ml.

Alternative SUMO1 matched antibody pairs: MP03279-1

Matched pair protein standard: Ag0414

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

• Capture antibody
• Detection antibody

• Catalog Number: 87942-4-PBS
• Tested Reactivity: human
• Immunogen: SUMO1 fusion protein Ag0414
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Full Name: SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
• GenBank accession number: BC006462
• Gene symbol: SUMO1
• Uniprot: P63165
• Gene ID (NCBI): 7341
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

• Catalog Number: 87942-2-PBS
• Tested Reactivity: human
• Immunogen: SUMO1 fusion protein Ag0414
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Full Name: SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
• GenBank accession number: BC006462
• Gene symbol: SUMO1
• Uniprot: P63165
• Gene ID (NCBI): 7341
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.