SUN2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SUN2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 27556-1-AP

Synonyms

UNC84B, SUN domain-containing protein 2, Sad1/unc-84 protein-like 2, Rab5-interacting protein, Protein unc-84 homolog B

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Validation Data Gallery

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  WB analysis using 27556-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27556-1-AP (SUN2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27556-1-AP

  WB result of SUN2 antibody (27556-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUN2 transfected HeLa cells.

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  WB analysis of mouse testis using 27556-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 27556-1-AP (SUN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 27556-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUN2 antibody (27556-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse testis tissue, Jurkat cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

27556-1-AP targets SUN2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SUN2 fusion protein Ag16077
• Full Name: unc-84 homolog B (C. elegans)
• Calculated Molecular Weight: 717 aa, 80 kDa
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC111717
• Gene Symbol: SUN2
• Gene ID (NCBI): 25777
• RRID: AB_2880906
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cancer Cell; Tumor cells impair immunological synapse formation via central nervous system-enriched metabolite; Authors - Yihong Li
human	WB	Virology; Long non-coding RNA SUN2-AS1 acts as a negative regulator of ISGs transcription to promote flavivirus infection; Authors - Chao Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nuo (Verified Customer) (01-28-2023)

There are several non specific bands nearby.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

SUN2 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:27556-1-AP)