SUNC1 Polyclonal antibody

SUNC1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26434-1-AP

Synonyms

SUN3, SUN domain-containing protein 3, Sad1/unc-84 domain-containing protein 1

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Validation Data Gallery

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  WB analysis of mouse testis using 26434-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26434-1-AP (SUNC1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 26434-1-AP

  IP result of anti-SUNC1 (IP:26434-1-AP, 4ug; Detection:26434-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IF Staining of HepG2 using 26434-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SUNC1 antibody (26434-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• CoIP: See 1 publications below

Product Information

26434-1-AP targets SUNC1 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SUNC1 fusion protein Ag23780
• Full Name: Sad1 and UNC84 domain containing 1
• Observed Molecular Weight: 37-41 kDa
• GenBank Accession Number: BC026189
• Gene Symbol: SUNC1
• Gene ID (NCBI): 256979
• RRID: AB_2880513
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUNC1 encodes SUN3 which is a member of SUN domain protein family, an evolutionary conserved family of inner nuclear membrane (INM) proteins that share a common C-terminal motif. SUN3 is mainly expressed in testis and is involved in sperm head formation.

Publications

Species	Application	Title
mouse	WB,CoIP	J Biol Chem; The testis-specific LINC component SUN3 is essential for sperm head shaping during mouse spermiogenesis.; Authors - Qian Gao
mouse	WB	Cell Mol Life Sci; CCDC28A deficiency causes sperm head defects, reduced sperm motility and male infertility in mice; Authors - Hongbin Zhou