Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26434-1-AP targets SUNC1 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23780 Product name: Recombinant human SUNC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-228 aa of BC026189 Sequence: MSGKTKARRAAMFFRRCSEDASGSASGNALLSEDENPDANGVTRSWKIILSTMLTLTFLLVGLLNHQWLKETDVPQKSRQLYAIIAEYGSRLYKYQARLRMPKEQLELLKKESQNLENNFRQILFLVEQIDVLKALLRDMKDGMDNNHNWNTHGDPVEDPDHTEEVSNLVNYVLKKLREDQVEMADYALKSAGASIIEAGTSESYKNNKAKLYWHGIGFLNHEMPPDI Predict reactive species |
| Full Name | Sad1 and UNC84 domain containing 1 |
| Observed Molecular Weight | 37-41 kDa |
| GenBank Accession Number | BC026189 |
| Gene Symbol | SUNC1 |
| Gene ID (NCBI) | 256979 |
| RRID | AB_2880513 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TAQ9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SUNC1 encodes SUN3 which is a member of SUN domain protein family, an evolutionary conserved family of inner nuclear membrane (INM) proteins that share a common C-terminal motif. SUN3 is mainly expressed in testis and is involved in sperm head formation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SUNC1 antibody 26434-1-AP | Download protocol |
| IP protocol for SUNC1 antibody 26434-1-AP | Download protocol |
| WB protocol for SUNC1 antibody 26434-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SUNC1 (SUN3) polyclonal antibody (Cat# 26434-1-AP) has 3 total citations. These appear in Ecotoxicology and Environmental Safety, Cell and Molecular Life Sciences, and Journal of Biological Chemistry. The research fields encompass sperm head shaping during spermiogenesis, CCDC28A-related male infertility, PFOA-induced reproductive toxicity, and the testis-specific LINC complex component SUN3. All cited applications were Western blot, with one also using CoIP, in mouse models.
| Species | Application | Title |
|---|---|---|
Ecotoxicol Environ Saf PFOA exposure decreases acrosome reaction and increases DNA oxidative damage by impairing the sperm head shaping in mice. | ||
Cell Mol Life Sci CCDC28A deficiency causes sperm head defects, reduced sperm motility and male infertility in mice | ||
J Biol Chem The testis-specific LINC component SUN3 is essential for sperm head shaping during mouse spermiogenesis. |
