SUPT16H Polyclonal antibody

SUPT16H Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 20551-1-AP

Synonyms

CDC68, FACT 140 kDa subunit, FACT complex subunit SPT16, FACT140, FACTP140, hSPT16, SPT16/CDC68, SUPT16H

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Validation Data Gallery

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  WB analysis of mouse brain using 20551-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20551-1-AP (SUPT16H antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20551-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20551-1-AP (SUPT16H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20551-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20551-1-AP (SUPT16H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 20551-1-AP

  IP result of anti-SUPT16H (IP:20551-1-AP, 4ug; Detection:20551-1-AP 1:1000) with mouse brain tissue lysate 3440ug.

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  IHC staining of human tonsillitis using 20551-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 20551-1-AP (SUPT16H antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human tonsillitis using 20551-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 20551-1-AP (SUPT16H antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 20551-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20551-1-AP (SUPT16H antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 20551-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20551-1-AP (SUPT16H antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 20551-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20551-1-AP (SUPT16H antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 20551-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20551-1-AP (SUPT16H antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human tonsillitis using 20551-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 20551-1-AP (SUPT16H antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human tonsillitis using 20551-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 20551-1-AP (SUPT16H antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human tonsillitis tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

20551-1-AP targets SUPT16H in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: suppressor of Ty 16 homolog (S. cerevisiae)
• Calculated Molecular Weight: 120 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_007192
• Gene Symbol: SUPT16H
• Gene ID (NCBI): 11198
• RRID: AB_10700005
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUPT16H, also named as FACT140, FACTP140, SPT16 and CDC68, belongs to the peptidase M24 family and SPT16 subfamily. SUPT16H is a component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). It also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. The antibody is specific to SUPT16H.

Publications

Species	Application	Title
		Arterioscler Thromb Vasc Biol; Novel Long Noncoding RNA, Macrophage Inflammation-Suppressing Transcript (MIST), Regulates Macrophage Activation During Obesity.; Authors - Kenneth Stapleton
mouse	IHC	FASEB J; Chronic exercise training activates histone turnover in mouse skeletal muscle fibers.; Authors - Ikumi Ohsawa
human	WB	J Mol Biol; Proteomic characterization of the nucleolar linker histone H1 interaction network.; Authors - Heather J Szerlong
human	WB,IHC	Biomed Pharmacother; CBL0137 activates ROS/BAX signaling to promote caspase-3/GSDME-dependent pyroptosis in ovarian cancer cells; Authors - Chang Yang