Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human brain tissue, human gliomas tissue, rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25606-1-AP targets SUPT7L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22264 Product name: Recombinant human SUPT7L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-414 aa of BC074000 Sequence: MNLQRYWGEIPISSSQTNRSSFDLLPREFRLVEVHDPPLHQPSANKPKPPTMLDIPSEPCSLTIHTIQLIQHNRRLRNLIATAQAQNQQQTEGVKTEESEPLPSCPGSPPLPDDLLPLDCKNPNAPFQIRHSDPESDFYRGKGEPVTELSWHSCRQLLYQAVATILAHAGFDCANESVLETLTDVAHEYCLKFTKLLRFAVDREARLGQTPFPDVMEQVFHEVGIGSVLSLQKFWQHRIKDYHSYMLQISKQLSEEYERIVNPEKATEDAKPVKIKEEPVSDITFPVSEELEADLASGDQSLPMGVLGAQSERFPSNLEVEASPQASSAEVNASPLWNLAHVKMEPQESEEGNVSGHGVLGSDVFEEPMSGMSEAGIPQSPDDSDSSYGSHSTDSLMGSSPVFNQRCKKRMRKI Predict reactive species |
| Full Name | suppressor of Ty 7 (S. cerevisiae)-like |
| Calculated Molecular Weight | 414 aa, 46 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC074000 |
| Gene Symbol | SUPT7L |
| Gene ID (NCBI) | 9913 |
| RRID | AB_2880156 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94864 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SUPT7L, also named as KIAA0764, STAF65gamma, is a component of the STAGA transcription coactivator-HAT complex. The MW of SUPT7L is 46-65 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SUPT7L antibody 25606-1-AP | Download protocol |
| IHC protocol for SUPT7L antibody 25606-1-AP | Download protocol |
| WB protocol for SUPT7L antibody 25606-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SUPT7L antibody (Cat# 25606-1-AP) has 1 citation. It was used in Western Blot (WB) for human samples in a study published in Human Genetics (2024), investigating loss-of-function variants in the STAGA complex component SUPT7L associated with a developmental disorder featuring generalized lipodystrophy. The research field encompasses human genetics and developmental biology.
| Species | Application | Title |
|---|---|---|
Hum Genet Loss-of-function variants affecting the STAGA complex component SUPT7L cause a developmental disorder with generalized lipodystrophy |
Reviews
What customers say
The single review for this product reports a 4-star rating from a Western Blot application using lymphoblastoid cells at a 1:1000 dilution. The reviewer noted the presence of multiple bands, questioning whether they were aspecific, but overall found the antibody worked well for their experiment.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Baptiste (Verified Customer) (08-28-2026) | Multiple bands (aspecific ?), work well
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