SUPT7L Polyclonal antibody

SUPT7L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25606-1-AP

Synonyms

Adenocarcinoma antigen ART1, ART1, KIAA0764, SPT7L, STAF65, STAF65(gamma), STAF65gamma, STAGA complex 65 subunit gamma, Suppressor of Ty 7 like, SUPT7L

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Validation Data Gallery

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  WB analysis of HeLa using 25606-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25606-1-AP (SUPT7L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 25606-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 25606-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 25606-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 25606-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 25606-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat brain using 25606-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 25606-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25606-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 25606-1-AP

  Immunofluorescent analysis of HeLa cells using 25606-1-AP (SUPT7L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human brain tissue, human gliomas tissue, rat brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 25606-1-AP is SUPT7L Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SUPT7L fusion protein Ag22264
• Full Name: suppressor of Ty 7 (S. cerevisiae)-like
• Calculated Molecular Weight: 414 aa, 46 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC074000
• Gene Symbol: SUPT7L
• Gene ID (NCBI): 9913
• RRID: AB_2880156
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUPT7L, also named as KIAA0764, STAF65gamma, is a component of the STAGA transcription coactivator-HAT complex. The MW of SUPT7L is 46-65 kDa.

Publications

Species	Application	Title
human	WB	Hum Genet; Loss-of-function variants affecting the STAGA complex component SUPT7L cause a developmental disorder with generalized lipodystrophy; Authors - Johannes Kopp