SUR1 Polyclonal antibody

SUR1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 55172-1-AP

Synonyms

ABC36, ABCC8, HHF1, HI, HRINS, MRP8, PHHI, Sulfonylurea receptor 1, SUR, SUR1, TNDM2

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Validation Data Gallery

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  WB analysis using 55172-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55172-1-AP (SUR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55172-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55172-1-AP (SUR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, NCI-H1299 cells, mouse brain tissue, BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55172-1-AP targets SUR1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 8
• Calculated Molecular Weight: 177 kDa
• Observed Molecular Weight: 140–177 kDa
• GenBank Accession Number: NM_000352
• Gene Symbol: ABCC8
• Gene ID (NCBI): 6833
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUR1 (Sulfonylurea receptor 1) is a member of the adenosine triphosphate (ATP)-binding cassette (ABC) protein superfamily, which encompasses a large group of membrane proteins that regulate the transport of ions and molecules across lipid bilayers (PMID:34769328). SUR1 regulates ATP-sensitive K+ channels and insulin release. Loss-of-function SUR1 mutations cause congenital hyperinsulinism and gain-of-function SUR1 mutations leading to neonatal diabetes (PMID: 18990670). SUR1 is recognized as a key mediator of central nervous system cellular swelling by the transient receptor potential melastatin 4 (TRPM4) channel. SUR1 (Sulfonylurea receptor 1) is a member of the adenosine triphosphate (ATP)-binding cassette (ABC) protein superfamily, which encompasses a large group of membrane proteins that regulate the transport of ions and molecules across lipid bilayers (PMID:34769328). SUR1 regulates ATP-sensitive K+ channels and insulin release. Loss-of-function SUR1 mutations cause congenital hyperinsulinism and gain-of-function SUR1 mutations leading to neonatal diabetes (PMID: 18990670). SUR1 is recognized as a key mediator of central nervous system cellular swelling by the transient receptor potential melastatin 4 (TRPM4) channel. SUR1 was detected 140-177 kDa in the pancreas, brain, heart (PMID: 34380876).