SURF1 Recombinant antibody, PBS Only (Detector)

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  IP experiment of HeLa using 83052-5-RR (same clone as 83052-5-PBS)

  IP result of anti-SURF1 (IP:83052-5-RR, 4ug; Detection:83052-5-RR 1:2000) with HeLa cells lysate 1050 ug. This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 83052-5-RR (same clone as 83052-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 83052-5-RR (same clone as 83052-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 83052-5-RR (same clone as 83052-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 83052-5-RR (same clone as 83052-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83052-5-RR (same clone as 83052-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SURF1 antibody (83052-5-RR, Clone: 230210F11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83052-5-RR (same clone as 83052-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SURF1 antibody (83052-5-RR, Clone: 230210F11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83052-5-RR (same clone as 83052-5-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug SURF1 Recombinant antibody (83052-5-RR, Clone:230210F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00034-3

  Cytometric bead array standard curve of MP00034-3, SURF1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83052-2-PBS. Detection antibody: 83052-5-PBS. Standard: Ag3959. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83052-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83052-5-RR against Human SURF1 were performed. The affinity constant is below 1 pM.

SURF1 Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Cat No. 83052-5-PBS

Clone No.230210F11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Synonyms

Surfeit locus protein 1, surfeit 1, SURF-1, SURF 1, 230210F11

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Product Information

83052-5-PBS targets SURF1 as part of a matched antibody pair:

MP00034-3: 83052-2-PBS capture and 83052-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SURF1 fusion protein Ag3959
• Full Name: surfeit 1
• Calculated Molecular Weight: 33 kDa
• GenBank Accession Number: BC028314
• Gene Symbol: SURF1
• Gene ID (NCBI): 6834
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15526
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.