IP experiment of HeLa using 83052-5-RR (same clone as 83052-5-PBS)
IP result of anti-SURF1 (IP:83052-5-RR, 4ug; Detection:83052-5-RR 1:2000) with HeLa cells lysate 1050 ug. This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
IP result of anti-SURF1 (IP:83052-5-RR, 4ug; Detection:83052-5-RR 1:2000) with HeLa cells lysate 1050 ug. This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 83052-5-RR (same clone as 83052-5-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 83052-5-RR (same clone as 83052-5-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 83052-5-RR (same clone as 83052-5-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 83052-5-RR (same clone as 83052-5-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
IF Staining of HeLa using 83052-5-RR (same clone as 83052-5-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SURF1 antibody (83052-5-RR, Clone: 230210F11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SURF1 antibody (83052-5-RR, Clone: 230210F11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
IF Staining of HeLa using 83052-5-RR (same clone as 83052-5-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SURF1 antibody (83052-5-RR, Clone: 230210F11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SURF1 antibody (83052-5-RR, Clone: 230210F11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
FC experiment of HepG2 using 83052-5-RR (same clone as 83052-5-PBS)
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug SURF1 Recombinant antibody (83052-5-RR, Clone:230210F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug SURF1 Recombinant antibody (83052-5-RR, Clone:230210F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 83052-5-RR
Biolayer interferometry (BLl) kinetic assays of 83052-5-RR against Human SURF1 were performed. The affinity constant is below 1 pM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
IHC staining of human liver cancer using 83052-5-RR (same clone as 83052-5-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 83052-5-RR (same clone as 83052-5-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 83052-5-RR (same clone as 83052-5-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 83052-5-RR (same clone as 83052-5-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83052-5-RR (SURF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
IP Figures
IP experiment of HeLa using 83052-5-RR (same clone as 83052-5-PBS)
IP result of anti-SURF1 (IP:83052-5-RR, 4ug; Detection:83052-5-RR 1:2000) with HeLa cells lysate 1050 ug. This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
IF/ICC Figures
IF Staining of HeLa using 83052-5-RR (same clone as 83052-5-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SURF1 antibody (83052-5-RR, Clone: 230210F11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
IF Staining of HeLa using 83052-5-RR (same clone as 83052-5-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SURF1 antibody (83052-5-RR, Clone: 230210F11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
FC (INTRA) Figures
FC experiment of HepG2 using 83052-5-RR (same clone as 83052-5-PBS)
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug SURF1 Recombinant antibody (83052-5-RR, Clone:230210F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83052-5-PBS in a different storage buffer formulation.
AFFINITY Figures
Affinity and Kinetic Characterization of 83052-5-RR
Biolayer interferometry (BLl) kinetic assays of 83052-5-RR against Human SURF1 were performed. The affinity constant is below 1 pM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
SURF1 Recombinant antibody
Catalog Number
83052-5-PBS
Citations
-
Dilutions
Applications
IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA
Reactivity
human, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
