Tested Applications
| Positive WB detected in | mouse brain tissue, mouse liver tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11599-1-AP targets SURF4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2175 Product name: Recombinant human SURF4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-269 aa of BC018741 Sequence: MGQNDLMGTAEDFADQFLRVTKQYLPHVARLCLISTFLEDGIRMWFQWSEQRDYIDTTWNCGYLLASSFVFLNLLGQLTGCVLVLSRNFVQYACFGLFGIIALQTIAYSILWDLKFLMRNLALGGGLLLLLAESRSEGKSMFAGVPTMRESSPKQYMQLGGRVLLVLMFMTLLHFDASFFSIVQNIVGTALMILVAIGFKTKLAALTLVVWLFAINVYFNAFWTIPVYKPMHDFLKYDFFQTMSVIGGLLLVVALGPGGVSMDEKKKEW Predict reactive species |
| Full Name | surfeit 4 |
| Calculated Molecular Weight | 269 aa, 30 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC018741 |
| Gene Symbol | SURF4 |
| Gene ID (NCBI) | 6836 |
| RRID | AB_2877782 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15260 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SURF4 antibody 11599-1-AP | Download protocol |
| WB protocol for SURF4 antibody 11599-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-SURF4 antibody (Cat# 11599-1-AP) has 4 citations across four journals: Journal of Translational Medicine, Cancer Immunology, Immunotherapy, Biochimica et Biophysica Acta – Molecular Cell Biology of Lipids, and Nan Fang Yi Ke Da Xue Xue Bao. Research fields span breast cancer prognosis, glioblastoma and pan-cancer therapeutic targets, lipid metabolism and liver injury in mice, and gastric cancer cell migration and proliferation. Applications include IHC and WB in human and mouse samples.
| Species | Application | Title |
|---|---|---|
J Transl Med Identifying prognostic markers in spatially heterogeneous breast cancer microenvironment | ||
Cancer Immunol Immunother Identification of SURF4 and RALGAPA1 as promising therapeutic targets in glioblastoma and pan-cancer through integrative multi-omics, CRISPR-Cas9 screening and prognostic meta-analysis | ||
Biochim Biophys Acta Mol Cell Biol Lipids Inducible global knockout of surfeit locus protein 4 in adult mice results in hypolipidemia, intestinal lipid accumulation, liver injury, and increased mortality
| ||
Nan Fang Yi Ke Da Xue Xue Bao [High expression of SURF4 promotes migration, invasion and proliferation of gastric cancer cells by inhibiting tight junction proteins]. |
Reviews
What customers say
The product received a 5-star rating from a user who employed it for immunofluorescence on HeLa cells. The reviewer reported a good, clear signal with minimal background at a 1:500 dilution, and noted that antigen retrieval was not required. Overall, the feedback highlights reliable performance and strong signal quality in IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Fiona (Verified Customer) (10-19-2021) | Used for immunofluorescence on HeLa cells. Good, clear signal with minimal background at 1:500 without antigen retrieval.
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