SURF4 Polyclonal antibody

SURF4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 11599-1-AP

Synonyms

ERV29, SURF 4, SURF4, surfeit 4, Surfeit locus protein 4

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Validation Data Gallery

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  WB analysis using 11599-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11599-1-AP (SURF4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 11599-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11599-1-AP (SURF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11599-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11599-1-AP (SURF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 11599-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11599-1-AP (SURF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11599-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11599-1-AP (SURF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse liver tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

11599-1-AP targets SURF4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SURF4 fusion protein Ag2175
• Full Name: surfeit 4
• Calculated Molecular Weight: 269 aa, 30 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC018741
• Gene Symbol: SURF4
• Gene ID (NCBI): 6836
• RRID: AB_2877782
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	J Transl Med; Identifying prognostic markers in spatially heterogeneous breast cancer microenvironment; Authors - Guohong Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Fiona (Verified Customer) (10-19-2021)

Used for immunofluorescence on HeLa cells. Good, clear signal with minimal background at 1:500 without antigen retrieval.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500