SURF6 Polyclonal antibody

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  WB analysis using 33693-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33693-1-AP (SURF6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

SURF6 Polyclonal Antibody for WB, ELISA

Cat No. 33693-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

SURF-6

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Tested Applications

• Positive WB detected in: Caco-2 cells, HL-60 cells, SW480 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33693-1-AP targets SURF6 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag38583

  Product name: Recombinant human SURF6 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-300 aa of BC006197

  Sequence: MASLLAKDAYLQSLAKKICSHSAPEQQARTRAGKTQGSETAGPPKKKRKKTQKKFRKREEKAAEHKAKSLGEKSPAASGARRPEAAKEEAAWASSSAGNPADGLATEPESVFALDVLRQRLHEKIQEARGQGSAKELSPAALEKRRRRKQERDRKKRKRKELRAKEKARKAEEATEAQEVVEATPEGACTEPREPPGLIFNKVEVSEDEPASKAQRRKEKRQRVKGNLTPLTGRNYRQLLERLQARQSRLDELRGQDEGKAQELEAKMKWTNLLYKAEGVKIRDDERLLQEALKRKEKRR

• Full Name: surfeit 6
• Calculated Molecular Weight: 361 aa, 41 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC006197
• Gene Symbol: SURF6
• Gene ID (NCBI): 6838
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O75683
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SURF6 has been described as a nucleolar protein that belongs to an evolutionary conserved protein family, which members are present in fungi, animal and plant kingdoms but are absent in bacteria. The characteristic feature of the SURF6 proteins is the presence of a conservative domain positioned in the carboxyl terminus that has an average residue identity of 36% between different species and is enriched with lysine and arginine residues, which confer its overall basic charge (pI > 10). (PMID: 28873013)