Tested Applications
| Positive WB detected in | HCT 116 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse small intestine tissue, C6 cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10574-1-AP targets SUV39H1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0854 Product name: Recombinant human SUV39H1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 7-277 aa of BC006238 Sequence: GCSVCCKSSWNQLQDLCRLAKLSCPALGISKRNLYDFEVEYLCDYKKIREQEYYLVKWRGYPDSESTWEPRQNLKCVRILKQFHKDLERELLRRHHRSKTPRHLDPSLANYLVQKAKQRRALRRWEQELNAKRSHLGRITVENEVDLDGPPRAFVYINEYRVGEGITLNQVAVGCECQDCLWAPTGGCCPGASLHKFAYNDQGQVRLRAGLPIYECNSRCRCGYDCPNRVVQKGIRYDLCIFRTDDGRGWGVRTLEKIRKNSFVMEYVGEI Predict reactive species |
| Full Name | suppressor of variegation 3-9 homolog 1 (Drosophila) |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 48-50 kDa |
| GenBank Accession Number | BC006238 |
| Gene Symbol | SUV39H1 |
| Gene ID (NCBI) | 6839 |
| RRID | AB_2196710 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43463 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Histone-lysine N-methyltransferase (SUV39H1), a nuclear protein, moves to the centromeres during mitosis and specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. It plays a vital role in heterochromatin organization, chromosome segregation, and mitotic progression. SUV39H1 has 2 isoforms with molecular weights of 48 and 49 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for SUV39H1 antibody 10574-1-AP | Download protocol |
| WB protocol for SUV39H1 antibody 10574-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SUV39H1 Polyclonal antibody (Cat# 10574-1-AP) has 24 citations published in journals including Nature Communications, Cell Metabolism, Nucleic Acids Research, Advanced Science, Cell Reports, Cell Death & Disease, Journal of Biological Chemistry, Oncotarget, iScience, and others. Research fields span epigenetics and histone modification, cancer biology (cholangiocarcinoma, breast, ovarian, prostate, glioblastoma, and other cancers), stem cell biology, virology (HIV, EBV), cardiovascular disease, and metabolism.
| Species | Application | Title |
|---|---|---|
Cell Metab Pregnenolone promotes immune evasion through blocking endogenous retrovirus expression. | ||
Nat Commun The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.
| ||
Nat Commun Improving erectile function in diabetic male mice by rescuing depalmitoylated FBP1 to reduce cavernosal lactate. | ||
Nucleic Acids Res The ZBTB24-CDCA7-HELLS axis suppresses the totipotent 2C-like reprogramming by maintaining Dux methylation and repression | ||
Nucleic Acids Res ATM, KAP1 and the Epstein-Barr virus polymerase processivity factor direct traffic at the intersection of transcription and replication | ||
J Exp Clin Cancer Res CyclinD1 inhibits dicer and crucial miRNA expression by chromatin modification to promote the progression of intrahepatic cholangiocarcinoma. |
Reviews
What customers say
The single review for 10574-1-AP is a 5-star rating from a Western Blot application on HeLa cells. The reviewer describes it as a great antibody for detecting SUV39H1 from HeLa extracts, using a 1/1000 dilution with an overnight incubation at 4°C. Overall, the feedback is positive, highlighting reliable performance and strong detection in their WB protocol.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Roy (Verified Customer) (06-15-2024) | Great antibody for detecting SUV39H1 from HeLa extracts (1/1000 dilution, overnight incubation at 4°C)
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