SUV39H1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SUV39H1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 10574-1-AP

Synonyms

H3 K9 HMTase 1, KMT1A, Lysine N methyltransferase 1A, MG44, Su(var)3 9 homolog 1, SUV39H, SUV39H1

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Validation Data Gallery

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  WB analysis using 10574-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10574-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 10574-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 10574-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10574-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10574-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10574-1-AP

  IP result of anti-SUV39H1 (IP:10574-1-AP, 4ug; Detection:10574-1-AP 1:500) with HeLa cells lysate 2800ug.

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Tested Applications

• Positive WB detected in: HCT 116 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse small intestine tissue, C6 cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 13 publications below

Product Information

10574-1-AP targets SUV39H1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SUV39H1 fusion protein Ag0854
• Full Name: suppressor of variegation 3-9 homolog 1 (Drosophila)
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC006238
• Gene Symbol: SUV39H1
• Gene ID (NCBI): 6839
• RRID: AB_2196710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histone-lysine N-methyltransferase (SUV39H1), a nuclear protein, moves to the centromeres during mitosis and specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. It plays a vital role in heterochromatin organization, chromosome segregation, and mitotic progression. SUV39H1 has 2 isoforms with molecular weights of 48 and 49 kDa.

Publications

Species	Application	Title
		Nucleic Acids Res; ATM, KAP1 and the Epstein-Barr virus polymerase processivity factor direct traffic at the intersection of transcription and replication; Authors - Huanzhou Xu
mouse	WB	Nat Commun; The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.; Authors - Guang Yang ; KO Validated
human	WB	J Med Chem; Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.; Authors - Ting Peng
human	WB	Cell Death Dis; Dicer promotes tumorigenesis by translocating to nucleus to promote SFRP1 promoter methylation in cholangiocarcinoma cells.; Authors - Wenlong Cheng
human	WB	Oncotarget; Downregulation of HP1α suppresses proliferation of cholangiocarcinoma by restoring SFRP1 expression.; Authors - Wenlong Cheng
human	WB	iScience; Linkage of methionine addiction, histone lysine hypermethylation, and malignancy.; Authors - Jun Yamamoto