SUZ12 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 84895-3-RR (same clone as 84895-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84895-3-RR (SUZ12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84895-3-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 84895-3-RR (same clone as 84895-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUZ12 antibody (84895-3-RR, Clone: 242491B3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84895-3-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 84895-3-RR (same clone as 84895-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SUZ12 antibody (84895-3-RR, Clone: 242491B3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84895-3-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 84895-3-RR (same clone as 84895-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SUZ12 Recombinant antibody (84895-3-RR, Clone:242491B3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84895-3-PBS in a different storage buffer formulation.

SUZ12 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 84895-3-PBS

Clone No.242491B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

KIAA0160, Joined to JAZF1 protein, JJAZ1, CHET9, ChET 9 protein

---

Product Information

84895-3-PBS targets SUZ12 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: suppressor of zeste 12 homolog (Drosophila)
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: NM_015355
• Gene Symbol: SUZ12
• Gene ID (NCBI): 23512
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q15022
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SUZ12, also termed CHET9, JJAZ1 or KIAA0160, belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. It is a polycomb group (PcG) protein. SUZ12 is a component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors.